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Sökning: WFRF:(Pipaliya Shweta V) > Combined nanometric...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004883naa a2200517 4500
001oai:DiVA.org:uu-486329
003SwePub
008221006s2022 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4863292 URI
0247 a https://doi.org/10.1186/s12915-022-01402-32 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Santos, Ruiu Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland.;Univ Zurich, Inst Anat, Winterthurerstr 190, CH-8057 Zurich, Switzerland.4 aut
2451 0a Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada
264 c 2022-09-21
264 1b BioMed Central (BMC),c 2022
338 a electronic2 rdacarrier
520 a Background Giardia lamblia, a parasitic protist of the Metamonada supergroup, has evolved one of the most diverged endocytic compartment systems investigated so far. Peripheral endocytic compartments, currently known as peripheral vesicles or vacuoles (PVs), perform bulk uptake of fluid phase material which is then digested and sorted either to the cell cytosol or back to the extracellular space. Results Here, we present a quantitative morphological characterization of these organelles using volumetric electron microscopy and super-resolution microscopy (SRM). We defined a morphological classification for the heterogenous population of PVs and performed a comparative analysis of PVs and endosome-like organelles in representatives of phylogenetically related taxa, Spironucleus spp. and Tritrichomonas foetus. To investigate the as-yet insufficiently understood connection between PVs and clathrin assemblies in G. lamblia, we further performed an in-depth search for two key elements of the endocytic machinery, clathrin heavy chain (CHC) and clathrin light chain (CLC), across different lineages in Metamonada. Our data point to the loss of a bona fide CLC in the last Fornicata common ancestor (LFCA) with the emergence of a protein analogous to CLC (GlACLC) in the Giardia genus. Finally, the location of clathrin in the various compartments was quantified. Conclusions Taken together, this provides the first comprehensive nanometric view of Giardia's endocytic system architecture and sheds light on the evolution of GlACLC analogues in the Fornicata supergroup and, specific to Giardia, as a possible adaptation to the formation and maintenance of stable clathrin assemblies at PVs.
650 7a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
653 a Endocytosis
653 a Super-resolution microscopy (SRM)
653 a Volumetric electron microscopy
653 a Peripheral endocytic compartments (PECs)
653 a Convergent evolution
653 a Metamonada
653 a Giardia
653 a Tritrichomonas
653 a Spironucleus
653 a Peripheral vacuoles
7001 a Astvaldsson, Asgeir,d 1981-u Uppsala universitet,Institutionen för cell- och molekylärbiologi,Natl Vet Inst, Dept Microbiol, S-75123 Uppsala, Sweden.4 aut0 (Swepub:uu)asgas474
7001 a Pipaliya, Shweta, Vu Univ Alberta, Dept Med, Div Infect Dis, Edmonton, AB, Canada.;Ecole Polytech Fed Lausanne, Sch Life Sci, Lausanne, Switzerland.;Swiss Inst Bioinformat, Lausanne, Switzerland.4 aut
7001 a Zumthor, Jon Paulinu Amt Lebensmittelsicherheit & Tiergesundheit Graub, Chur, Switzerland.4 aut
7001 a Dacks, Joel B.u Univ Alberta, Dept Med, Div Infect Dis, Edmonton, AB, Canada.;CAS, Biol Ctr, Inst Parasitol, Branisovska 31, Ceske Budejovice 37005, Czech Republic.4 aut
7001 a Svärd, Staffanu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)stsva021
7001 a Hehl, Adrian B.u Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland.4 aut
7001 a Faso, Carmenu Univ Bern, Inst Cell Biol, Bern, Switzerland.;Univ Bern, Multidisciplinary Ctr Infect Dis, Vetsuisse, Bern, Switzerland.4 aut
7102 a Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland.;Univ Zurich, Inst Anat, Winterthurerstr 190, CH-8057 Zurich, Switzerland.b Institutionen för cell- och molekylärbiologi4 org
7730 t BMC Biologyd : BioMed Central (BMC)g 20:1q 20:1x 1741-7007
8564 u https://doi.org/10.1186/s12915-022-01402-3y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1701643/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-486329
8564 8u https://doi.org/10.1186/s12915-022-01402-3

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