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Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models

Topping, Louise M. (author)
University of Oxford,Kennedy Institute of Rheumatology
Romero-Castillo, Laura (author)
Karolinska Institutet,Karolinska Institute
Urbonaviciute, Vilma (author)
Karolinska Institutet,Karolinska Institute
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Bolinsson, Hans (author)
Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
Clanchy, Felix I. (author)
University of Oxford,Kennedy Institute of Rheumatology
Holmdahl, Rikard (author)
Karolinska Institutet,Karolinska Institute
Bäckström, B. Thomas (author)
Lund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,BTB Emulsions AB
Williams, Richard O. (author)
University of Oxford,Kennedy Institute of Rheumatology
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ISSN 1664-3224
2022-06-13
2022
English.
In: Frontiers in Immunology. - Stockholm : Frontiers Media SA. - 1664-3224. ; 13
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for in vivo research involving animal models of autoimmune disease and in vaccine development.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Keyword

animal disease models
antigen emulsions
autoimmunity
CIA
Complete Freund’s Adjuvant
EAE
emulsification

Publication and Content Type

art (subject category)
ref (subject category)

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