WFRF:(Sabeti Pardis C.)
Sökning: WFRF:(Sabeti Pardis C.) > Strand-specific RNA...
- Broadbent, Kate M. (författare)
Strand-specific RNA sequencing in Plasmodium falciparum malaria identifies developmentally regulated long non-coding RNA and circular RNA
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
- 2015-06-13
- Springer Science and Business Media LLC,2015
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:uu-257643
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-257643URI
- https://doi.org/10.1186/s12864-015-1603-4DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Background: The human malaria parasite Plasmodium falciparum has a complex and multi-stage life cycle that requires extensive and precise gene regulation to allow invasion and hijacking of host cells, transmission, and immune escape. To date, the regulatory elements orchestrating these critical parasite processes remain largely unknown. Yet it is becoming increasingly clear that long non-coding RNAs (lncRNAs) could represent a missing regulatory layer across a broad range of organisms. Results: To investigate the regulatory capacity of lncRNA in P. falciparum, we harvested fifteen samples from two time-courses. Our sample set profiled 56 h of P. falciparum blood stage development. We then developed and validated strand-specific, non-polyA-selected RNA sequencing methods, and pursued the first assembly of P. falciparum strand-specific transcript structures from RNA sequencing data. This approach enabled the annotation of over one thousand lncRNA transcript models and their comprehensive global analysis: coding prediction, periodicity, stage-specificity, correlation, GC content, length, location relative to annotated transcripts, and splicing. We validated the complete splicing structure of three lncRNAs with compelling properties. Non-polyA-selected deep sequencing also enabled the prediction of hundreds of intriguing P. falciparum circular RNAs, six of which we validated experimentally. Conclusions: We found that a subset of lncRNAs, including all subtelomeric lncRNAs, strongly peaked in expression during invasion. By contrast, antisense transcript levels significantly dropped during invasion. As compared to neighboring mRNAs, the expression of antisense-sense pairs was significantly anti-correlated during blood stage development, indicating transcriptional interference. We also validated that P. falciparum produces circRNAs, which is notable given the lack of RNA interference in the organism, and discovered that a highly expressed, five-exon antisense RNA is poised to regulate P. falciparum gametocyte development 1 (PfGDV1), a gene required for early sexual commitment events.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
- RNA sequencing
- Non-coding RNA
- lncRNA
- Antisense RNA
- circRNA
- microRNA
- Malaria
- Plasmodium
- Transcriptome
- Gene regulation
- Extreme genome
- PfGDV1
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Broadbent, Jill C. (författare)
- Ribacke, UlfUppsala universitet,Mikrobiologi(Swepub:uu)ulfri962 (författare)
- Wirth, Dyann (författare)
- Rinn, John L. (författare)
- Sabeti, Pardis C. (författare)
- Uppsala universitetMikrobiologi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:BMC Genomics: Springer Science and Business Media LLC161471-2164
Internetlänk
- https://doi.org/10.1186/s12864-015-1603-4Fulltext
- https://uu.diva-portal.org/smash/get/diva2:840658/FULLTEXT01.pdffulltext:print
- https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-015-1603-4
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-257643
- https://doi.org/10.1186/s12864-015-1603-4
Hitta mer i SwePub
- Av författaren/redakt...
- Broadbent, Kate ...
- Broadbent, Jill ...
- Ribacke, Ulf
- Wirth, Dyann
- Rinn, John L.
- Sabeti, Pardis C ...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Medicinsk biotek ...
- och Medicinsk biotek ...
- Artiklar i publikationen
- BMC Genomics
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
