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Protein structural ...
Protein structural analysis by cryogenic electron microscopy
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- Hall, Michael, 1980- (författare)
- Umeå universitet,Kemiska institutionen
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- Schexnaydre, Erin (författare)
- Umeå universitet,Kemiska institutionen
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- Holmlund, Camilla (författare)
- Umeå universitet,Kemiska institutionen
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- Carroni, Marta (författare)
- SciLifeLab Cryo-EM Facility, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden
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(creator_code:org_t)
- 1
- New York : Humana Press, 2023
- 2023
- Engelska.
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Ingår i: Advanced methods in structural biology. - New York : Humana Press. - 9781071631461 - 9781071631478 ; , s. 439-463
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cryogenic electron microscopy (cryo-EM) is constantly developing and growing as a major technique for structure determination of protein complexes. Here, we detail the first steps of any cryo-EM project: specimen preparation and data collection. Step by step, a list of material needed is provided and the sequence of actions to carry out is given. We hope that these protocols will be useful to all people getting started with cryo-EM.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Annan kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Other Chemistry Topics (hsv//eng)
Nyckelord
- Cryo electron microscopy
- Protein structure
- Single particle analysis
- Vitrification
Publikations- och innehållstyp
- ref (ämneskategori)
- kap (ämneskategori)
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