Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis

Blaschek, LeonardStockholms universitet,Institutionen för ekologi, miljö och botanik,Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, Stockholm, Sweden (författare)

Vascular plants reinforce the cell walls of the different xylem cell types with lignin phenolic polymers. Distinct lignin chemistries differ between each cell wall layer and each cell type to support their specific functions. Yet the mechanisms controlling the tight spatial localization of specific lignin chemistries remain unclear. Current hypotheses focus on control by monomer biosynthesis and/or export, while cell wall polymerization is viewed as random and nonlimiting. Here, we show that combinations of multiple individual laccases (LACs) are nonredundantly and specifically required to set the lignin chemistry in different cell types and their distinct cell wall layers. We dissected the roles of Arabidopsis thaliana LAC4, 5, 10, 12, and 17 by generating quadruple and quintuple loss-of-function mutants. Loss of these LACs in different combinations led to specific changes in lignin chemistry affecting both residue ring structures and/or aliphatic tails in specific cell types and cell wall layers. Moreover, we showed that LAC-mediated lignification has distinct functions in specific cell types, waterproofing fibers, and strengthening vessels. Altogether, we propose that the spatial control of lignin chemistry depends on different combinations of LACs with nonredundant activities immobilized in specific cell types and cell wall layers.

Murozuka, EmikoUmeå universitet,Stockholms universitet,Institutionen för ekologi, miljö och botanik,Umeå University, Sweden,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, Stockholm, Sweden(Swepub:umu)emmu0009 (författare)
Serk, Henrik,1980-Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)hese0007 (författare)
Ménard, DelphineUmeå universitet,Stockholms universitet,Institutionen för ekologi, miljö och botanik,Umeå University, Sweden,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, Stockholm, Sweden(Swepub:umu)deme0005 (författare)
Pesquet, Edouard,1975-Umeå universitet,Stockholms universitet,Institutionen för ekologi, miljö och botanik,Bolincentret för klimatforskning (tills m KTH & SMHI),Umeå University, Sweden,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, Stockholm, Sweden; Bolin Centre for Climate Research, Stockholm University, Stockholm, Sweden(Swepub:umu)edpe0005 (författare)
Stockholms universitetInstitutionen för ekologi, miljö och botanik (creator_code:org_t)

Ingår i:The Plant Cell: Oxford University Press (OUP)35:2, s. 889-9091040-46511532-298X

Av författaren/redakt...: Blaschek, Leonar ...; Murozuka, Emiko; Serk, Henrik, 19 ...; Ménard, Delphine; Pesquet, Edouard ...

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