Sökning: WFRF:(Thomas Anub M.) >
Combined Inhibition...
Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome.
-
- Thomas, Anub Mathew (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway
-
- Schjalm, Camilla (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway
-
- Nilsson, Per H., 1980- (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Oslo University Hospital, Norway;University of Oslo, Norway,Linnaeus Ctr Biomat Chem, BMC;HoRB
-
visa fler...
-
- Lindenskov, Paal H H (författare)
- Oslo University Hospital, Norway
-
- Rørtveit, Runa (författare)
- Norwegian University of Life Sciences, Norway
-
- Solberg, Rønnaug (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway
-
- Saugstad, Ola Didrik (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway
-
- Berglund, Magnus M (författare)
- Swedish Orphan Biovitrum, Sweden
-
- Strömberg, Patrik (författare)
- Swedish Orphan Biovitrum, Sweden
-
- Lau, Corinna (författare)
- Nordland Hospital, Norway
-
- Espevik, Terje (författare)
- Norwegian University of Science and Technology, Norway
-
- Jansen, Johan Høgset (författare)
- Norwegian University of Life Sciences, Norway
-
- Castellheim, Albert (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård,Institute of Clinical Sciences, Department of Anesthesiology and Intensive care
-
- Mollnes, Tom Eirik (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway;Nordland Hospital, Norway;Norwegian University of Science and Technology, Norway;University of Tromsø, Norway
-
- Barratt-Due, Andreas (författare)
- Oslo University Hospital, Norway;University of Oslo, Norway
-
visa färre...
-
(creator_code:org_t)
- 2018-02-27
- 2018
- Engelska.
-
Ingår i: Neonatology. - : S. Karger. - 1661-7800 .- 1661-7819. ; 113:4, s. 322-330
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://www.karger.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://gup.ub.gu.se...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Meconium aspiration syndrome (MAS) is a severe lung condition affecting newborns and it can lead to a systemic inflammatory response. We previously documented complement activation and cytokine release in a piglet MAS model. Additionally, we showed ex vivo that meconium-induced inflammation was dependent on complement and Toll-like receptors.OBJECTIVES: To assess the efficacy of the combined inhibition of complement (C5) and CD14 on systemic inflammation induced in a forceful piglet MAS model.METHODS: Thirty piglets were randomly allocated to a treatment group receiving the C5-inhibitor SOBI002 and anti-CD14 (n = 15) and a nontreated control group (n = 15). MAS was induced by intratracheal meconium instillation, and the piglets were observed for 5 h. Complement, cytokines, and myeloperoxidase (MPO) were measured by ELISA.RESULTS: SOBI002 ablated C5 activity and the formation of the terminal complement complex in vivo. The combined inhibition attenuated the inflammasome cytokines IL-1β and IL-6 by 60 (p = 0.029) and 44% (p = 0.01), respectively, and also MPO activity in the bronchoalveolar fluid by 42% (p = 0.017). Ex vivo experiments in human blood revealed that the combined regimen attenuated meconium-induced MPO release by 64% (p = 0.008), but there was only a negligible effect with single inhibition, indicating a synergic cross-talk between the key molecules C5 and CD14.CONCLUSION: Combined inhibition of C5 and CD14 attenuates meconium-induced inflammation in vivo and this could become a future therapeutic regimen for MAS.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- C5
- CD14
- Complement
- Innate immunity
- Meconium aspiration syndrome
- Systemic inflammation
- Toll-like receptor
- Medicin
- Medicine
- C5
- Cd14
- Complement
- Innate immunity
- Meconium aspiration syndrome
- Systemic inflammation
- Toll-like receptor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Thomas, Anub Mat ...
-
Schjalm, Camilla
-
Nilsson, Per H., ...
-
Lindenskov, Paal ...
-
Rørtveit, Runa
-
Solberg, Rønnaug
-
visa fler...
-
Saugstad, Ola Di ...
-
Berglund, Magnus ...
-
Strömberg, Patri ...
-
Lau, Corinna
-
Espevik, Terje
-
Jansen, Johan Hø ...
-
Castellheim, Alb ...
-
Mollnes, Tom Eir ...
-
Barratt-Due, And ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Lungmedicin och ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Immunologi inom ...
- Artiklar i publikationen
-
Neonatology
- Av lärosätet
-
Linnéuniversitetet
-
Göteborgs universitet