Sökning: WFRF:(Thorlin Thorleif)
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Endothelin-1 decrea...
Endothelin-1 decreases glutamate uptake in primary cultured rat astrocytes.
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- Leonova, Julia (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Thorlin, Thorleif, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Åberg, N David, 1970 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap,Institute of Clinical Neurosciences, Section of Experimental Neuroscience
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visa fler...
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- Eriksson, Peter S, 1959 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Rönnbäck, Lars, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Hansson, Elisabeth, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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visa färre...
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: American journal of physiology. Cell physiology. - 0363-6143. ; 281:5
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Endothelin-1 (ET-1) is a potent vasoconstrictor peptide that is also known to induce a wide spectrum of biological responses in nonvascular tissue. In this study, we found that ET-1 (100 nM) inhibited the glutamate uptake in cultured astrocytes expressing the glutamate/aspartate transporter (GLAST); astrocytes did not express the glutamate transporter-1 (GLT-1). The V(max) and the K(m) of the glutamate uptake were reduced by 57% and 47%, respectively. Application of the ET(A) and ET(B) receptor antagonists BQ-123 and BQ-788 partly inhibited the ET-1-evoked decrease in the glutamate uptake, whereas the nonspecific ET receptor antagonist bosentan completely inhibited this decrease. Incubation of the cultures with pertussis toxin abolished the effect of ET-1 on the uptake. The ET-1-induced decrease in the glutamate uptake was independent of extracellular free Ca(2+) concentration, whereas the intracellular Ca(2+) antagonists thapsigargin and 3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester abolished the effect of ET-1 on the glutamate uptake. Incubation with the protein kinase C (PKC) antagonist staurosporine, but not with the fatty acid-binding protein bovine serum albumin, prevented the ET-1-induced decrease in the glutamate uptake. These results suggest that ET-1 impairs the high-affinity glutamate uptake in cultured astrocytes through a G protein-coupled mechanism, involving PKC and changes in intracellular Ca(2+).
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- Amino Acid Sequence
- Animals
- Astrocytes
- drug effects
- metabolism
- Barium Compounds
- pharmacology
- Blotting
- Western
- Cells
- Cultured
- Cerebral Cortex
- cytology
- drug effects
- metabolism
- Chlorides
- pharmacology
- Depression
- Chemical
- Endothelin-1
- pharmacology
- Glutamic Acid
- metabolism
- Immunohistochemistry
- Molecular Sequence Data
- Rats
- Rats
- Sprague-Dawley
- Transduction
- Genetic
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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