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Microvilli structur...
Microvilli structures on B lymphocytes: inducible functional domains?
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Greicius, Gediminas (författare)
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- Westerberg, Lisa (författare)
- Karolinska Institutet
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- Davey, Edward J (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory,Research Group of Clinical Molecular Genetics
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Buentke, Eva (författare)
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- Scheynius, Annika (författare)
- Karolinska Institutet
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- Thyberg, Johan (författare)
- Karolinska Institutet
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Severinson, Eva (författare)
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(creator_code:org_t)
- Oxford University Press (OUP), 2004
- 2004
- Engelska.
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Ingår i: Int Immunol. - : Oxford University Press (OUP). - 0953-8178 .- 1460-2377. ; 16:2, s. 353-64
- Relaterad länk:
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http://www.ncbi.nlm....
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https://academic.oup...
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https://urn.kb.se/re...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Interactive contact between B lymphocytes and T cells is necessary for their expansion during an immune response. It has been shown that B lymphocytes receive signals from T cells, such as IL-4 and cross-linking of CD40, which are crucial for their differentiation. We previously found that these factors induce formation of microvilli on B cells and that this was correlated with increased homotypic adhesion of B lymphocytes. In this study we have investigated if IL-4 induce segregation of proteins to microvilli and lipid rafts. Using immuno-electron microscopy we analyzed cell-surface distribution of molecules involved in B-T cell co-activation. Recruitment to detergent-resistant membrane fractions was analyzed using sucrose gradient centrifugation. We found that microvilli were enriched in ICAM-1 and MHC class II molecules. In contrast, LFA-1 and CD40 were more abundant on the smooth cell surfaces, while B7-2 (CD86) was randomly distributed. We also discovered that depletion of cholesterol, using beta-methyl-cyclodextrin, lowered the number of microvilli, indicating that intact lipid rafts are required for their expression. Moreover, activation of B lymphocytes by lipopolysaccharide (LPS) induced increased expression of GM(1), a marker for lipid rafts. However, although both surface and total levels of GM(1) were similar in B lymphocytes stimulated with either LPS or LPS plus IL-4, GM(1) was mainly expressed on microvilli in LPS plus IL-4-stimulated cells. Taken together, our results indicate that microvilli represent distinct inducible membrane domains that can regulate direct cell-cell interactions via grouping and three-dimensional presentation of cell-surface receptors.
Nyckelord
- Animals
- Antigens; CD/metabolism
- Antigens; CD40/metabolism
- B-Lymphocytes/immunology/*ultrastructure
- Cell Communication/drug effects/physiology
- Cholera Toxin/pharmacology
- Cyclodextrins/pharmacology
- Genes; MHC Class II/*physiology
- Intercellular Adhesion Molecule-1/*metabolism
- Interleukin-4/pharmacology
- Lipopolysaccharides/pharmacology
- Lymphocyte Activation/drug effects
- Lymphocyte Function-Associated Antigen-1/metabolism
- Membrane Glycoproteins/metabolism
- Membrane Microdomains/metabolism/*ultrastructure
- Mice
- Microvilli/immunology/*ultrastructure
- Research Support; Non-U.S. Gov't
- T-Lymphocytes/immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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