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Sökning: onr:"swepub:oai:lup.lub.lu.se:0e80b0b2-af49-4a08-b826-ca77bbd8819a" > Rapid microwave-sti...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003489naa a2200433 4500
001oai:lup.lub.lu.se:0e80b0b2-af49-4a08-b826-ca77bbd8819a
003SwePub
008230210s1997 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/0e80b0b2-af49-4a08-b826-ca77bbd8819a2 URI
0247 a https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-92 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Ruijter, Emiel Thu Radboud University Medical Center4 aut
2451 0a Rapid microwave-stimulated fixation of entire prostatectomy specimens
264 1c 1997
520 a Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
653 a Fixation
653 a Immunohistochemistry
653 a Microwaves
653 a Prostate
7001 a Miller, Gary J.u University of Colorado4 aut
7001 a Aalders, Tilly W.u Radboud University Medical Center4 aut
7001 a Van De Kaa, Christina A.u Radboud University Medical Center4 aut
7001 a Schalken, Jack A.u Radboud University Medical Center4 aut
7001 a Debruyne, Frans M.u Radboud University Medical Center4 aut
7001 a Boon, Mathilde E.u Leiden Cytology and Pathology Laboratory4 aut
7001 a Schröder, F. H.4 cre
7001 a Lilja, H.u Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups4 cre0 (Swepub:lu)klke-hli
7001 a Robson, C. N.4 cre
7102 a Radboud University Medical Centerb University of Colorado4 org
7730 t Journal of Pathologyg 183:3, s. 369-375q 183:3<369-375x 0022-3417
8564 u http://dx.doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9y FULLTEXT
8564 8u https://lup.lub.lu.se/record/0e80b0b2-af49-4a08-b826-ca77bbd8819a
8564 8u https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9

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