WFRF:(Wikner Johan 1961 )
Sökning: WFRF:(Wikner Johan 1961 ) > (2000-2004) > Limited resolution ...
LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02410naa a2200361 4500 | |
| 001 | oai:DiVA.org:umu-52543 | |
| 003 | SwePub | |
| 008 | 120224s2003 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-525432 URI |
| 024 | 7 | a https://doi.org/10.1016/S0167-7012(03)00038-12 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Kisand, Veljo4 aut |
| 245 | 1 0 | a Limited resolution of 16S rDNA DGGE caused by melting properties and closely related DNA sequences |
| 264 | 1 | c 2003 |
| 338 | a print2 rdacarrier | |
| 520 | a The phylogenetic affiliation of 91 operational taxonomic units, randomly sampled from three aquatic microcosm experiments, was investigated by two PCR based and one culture dependent method. The occurrence of multiple melting domains and poor coupling between Tin and DGGE retardation was demonstrated to cause poor resolution at the species level in PCR-DGGE analysis of microbial communities. We also showed that the problem of multiple melting domains was particularly prone for brackish water bacterioplankton in the Flavobacterium genus, providing characteristic band morphology for this genus. Banding patterns from DGGE analysis may therefore be misinterpreted in terms of the species richness in natural bacterial communities, when using commonly applied universal primers. (C) 2003 Elsevier Science B.V. All rights reserved. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a 16S rDNA | |
| 653 | a flavobacteria | |
| 653 | a melting temperature | |
| 653 | a multiple melting domains | |
| 653 | a PCR-DGGE | |
| 653 | a phylogeny | |
| 700 | 1 | a Wikner, Johan,d 1961-u Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Johan Wikner4 aut0 (Swepub:umu)jowi0002 |
| 710 | 2 | a Umeå universitetb Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)4 org |
| 773 | 0 | t Journal of Microbiological Methodsg 54:2, s. 183-191q 54:2<183-191x 0167-7012x 1872-8359 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-52543 |
| 856 | 4 8 | u https://doi.org/10.1016/S0167-7012(03)00038-1 |
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