Photoderivatized polymer thin films at quartz crystal microbalance surfaces : Sensors for carbohydrate-protein interactions

• Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

functionalized perfluorophenyl azides

ligand-binding characteristics

lectin concanavalin-a

monomer/dimer equilibrium

glycolipid monolayers

plasmon resonance

mistletoe lectin

pisum-sativum

affinity

mannan

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)