Early Immune Responses to Borrelia garinii and Borrelia afzeliiin Lyme Borreliosis: Local Complement Activation in Erythema Migrans and in vitro Studies ofComplement Activation, Phagocytosis and Cytokine Profile

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Early Immune Responses to Borrelia garinii and Borrelia afzeliiin Lyme Borreliosis : Local Complement Activation in Erythema Migrans and in vitro Studies ofComplement Activation, Phagocytosis and Cytokine Profile

Sandholm, Kerstin (författare): School of Natural Sciences, Linneaus University, Kalmar

Henningsson, Anna J, 1972- (författare): Linköpings universitet,Infektionsmedicin,Hälsouniversitetet

Säve, Susanne (författare): School of Natural Sciences, Linneaus University, Kalmar

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Nordberg, Marika (författare): Linköpings universitet,Infektionsmedicin,Hälsouniversitetet

Garpmo, Ulf (författare): Department of Clinical Microbiology, Kalmar County Hospital, Sweden

Jansson, Christian (författare): The Åland Borrelia Group, the Åland Islands, Finland

Carlsson, Sten-Anders (författare): The Åland Borrelia Group, the Åland Islands, Finland

Nyman, Dag (författare): The Åland Borrelia Group, the Åland Islands, Finland

Bergström, Sven (författare): Department of Molecular Biology, Umeå University, Sweden

Forsberg, Pia (författare): Linköpings universitet,Infektionsmedicin,Hälsouniversitetet

Ernerudh, Jan (författare): Linköpings universitet,Klinisk immunologi,Hälsouniversitetet

Nilsson Ekdahl, Kristina (författare): School of Natural Sciences, Linneaus University, Kalmar

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Engelska.

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Abstract Ämnesord

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An optimal eradication of spirochetes in Lyme borreliosis depends on the early immune response, including the potent actions of the complement system. We here assessed possible differences between two Borrelia burgdorferi genospecies in their ability to activate complement, and the consequences of complement activation in terms of phagocytosis and induction of cytokines.Early local complement activation was assessed immunohistochemically in skin biopsies from patients with erythema migrans (EM) caused by B. afzelii or B. garinii. Complement activation, phagocytosis and early cytokine and chemokine release were studied in vitro by incubating clinical isolates of B. afzelii (K78 from a human skin biopsy) and B. garinii (LU59 from human cerebrospinal fluid) in human whole blood.B. afzelii and B. garinii were detected in skin biopsies from EM and deposition of C3-fragments and IgG was found adjacent to the spirochetes. In vitro, B. garinii LU59 induced higher complement activation (measured as the generation of C3a and C5b-9), while B. afzelii K78 recruited more factor H. Phagocytosis by granulocytes and monocytes was demonstrated to be largely dependent on complement activation since phagocytosis was substantially reduced by addition of the C3 inhibitor compstatin or a C5a receptor antagonist. The early cytokine and chemokine release in human blood in response to live spirochetes revealed a rapid and pronounced pro-inflammatory response, mainly associated with the Th1- and Th17-types.We conclude that complement is activated locally in the skin in EM, and that B. garinii LU59 activates complement more than B. afzelii K78. Complement activation is pivotal for efficient phagocytosis of Borrelia spirochetes, especially in early infection before specific antibodies are produced. Both B. garinii LU59 and B. afzelii K78 induce proinflammatory cytokines rapidly, but no clear differences were seen between the two genospecies in this respect.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Nyckelord

Lyme borreliosis
erythema migrans
inflammation
complement
cytokine
chemokine
phagocytosis

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Av författaren/redakt...: Sandholm, Kersti ...; Henningsson, Ann ...; Säve, Susanne; Nordberg, Marika; Garpmo, Ulf; Jansson, Christi ...; visa fler...; Carlsson, Sten-A ...; Nyman, Dag; Bergström, Sven; Forsberg, Pia; Ernerudh, Jan; Nilsson Ekdahl, ...; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Infektionsmedici ...

Av lärosätet: Linköpings universitet

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