Sökning: onr:"swepub:oai:DiVA.org:liu-206321" >
Efficient separatio...
Efficient separation of large particles and giant cancer cells using an isosceles trapezoidal spiral microchannel
-
- Park, Chanyong (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- Lim, Wanyoung (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- Song, Ryungeun (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
visa fler...
-
- Han, Jeonghun (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- You, Daeun (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- Kim, Sangmin (författare)
- Sungkyunkwan Univ, South Korea
-
- Lee, Jeong Eon (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- van Noort, Danny (författare)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
-
- Mandenius, Carl-Fredrik (författare)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
-
- Lee, Jinkee (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
- Hyun, Kyung-A. (författare)
- Yonsei Univ, South Korea
-
- Jung, Hyo-Il (författare)
- Yonsei Univ, South Korea
-
- Park, Sungsu (författare)
- Sungkyunkwan Univ SKKU, South Korea
-
visa färre...
-
(creator_code:org_t)
- 2024
- 2024
- Engelska.
-
Ingår i: The Analyst. - : ROYAL SOC CHEMISTRY. - 0003-2654 .- 1364-5528.
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors. Their size, however, complicates their isolation from mainstream tumor cell populations. Standard techniques like fluorescence-activated cell sorting (FACS) rely on fluorescent labeling, introducing potential challenges in subsequent PGCC analyses. In response, we developed the Isosceles Trapezoidal Spiral Microchannel (ITS mu C), a microfluidic device optimizing the Dean drag force (FD) and exploiting uniform vortices for enhanced separation. Numerical simulations highlighted ITS mu C's advantage in producing robust FD compared to rectangular and standard trapezoidal channels. Empirical results confirmed its ability to segregate larger polystyrene (PS) particles (avg. diameter: 50 mu m) toward the inner wall, while directing smaller ones (avg. diameter: 23 mu m) outward. Utilizing ITS mu C, we efficiently isolated PGCCs from doxorubicin-resistant triple-negative breast cancer (DOXR-TNBC) and patient-derived cancer (PDC) cells, achieving outstanding purity, yield, and viability rates (all greater than 90%). This precision was accomplished without fluorescent markers, and the versatility of ITS mu C suggests its potential in differentiating a wide range of heterogeneous cell populations. Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Park, Chanyong
-
Lim, Wanyoung
-
Song, Ryungeun
-
Han, Jeonghun
-
You, Daeun
-
Kim, Sangmin
-
visa fler...
-
Lee, Jeong Eon
-
van Noort, Danny
-
Mandenius, Carl- ...
-
Lee, Jinkee
-
Hyun, Kyung-A.
-
Jung, Hyo-Il
-
Park, Sungsu
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Biomedicinsk lab ...
- Artiklar i publikationen
-
The Analyst
- Av lärosätet
-
Linköpings universitet