Sökning: (WFRF:(Algethami Nader)) > CXCL2-CXCR2 axis me...
Fältnamn | Indikatorer | Metadata |
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000 | 05533naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:42d1fc68-d230-4713-84dd-d57b243183e2 | |
003 | SwePub | |
008 | 210621s2021 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/42d1fc68-d230-4713-84dd-d57b243183e22 URI |
024 | 7 | a https://doi.org/10.1007/s10585-021-10103-02 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Lepsenyi, Mattiasu Lund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)med-mli |
245 | 1 0 | a CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells |
264 | c 2021-06-11 | |
264 | 1 | b Springer Science and Business Media LLC,c 2021 |
520 | a Peritoneal metastasis is an insidious aspect of colorectal cancer. The aim of the present study was to define mechanisms regulating colon cancer cell adhesion and spread to peritoneal wounds after abdominal surgery. Mice was laparotomized and injected intraperitoneally with CT-26 colon carcinoma cells and metastatic noduli in the peritoneal cavity was quantified after treatment with a CXCR2 antagonist or integrin-αV-antibody. CT-26 cells expressed cell surface chemokine receptors CXCR2, CXCR3, CXCR4 and CXCR5. Stimulation with the CXCR2 ligand, CXCL2, dose-dependently increased proliferation and migration of CT-26 cells in vitro. The CXCR2 antagonist, SB225002, dose-dependently decreased CXCL2-induced proliferation and migration of colon cancer cells in vitro. Intraperitoneal administration of CT-26 colon cancer cells resulted in wide-spread growth of metastatic nodules at the peritoneal surface of laparotomized animals. Laparotomy increased gene expression of CXCL2 at the incisional line. Pretreatment with CXCR2 antagonist reduced metastatic nodules by 70%. Moreover, stimulation with CXCL2 increased CT-26 cell adhesion to extracellular matrix (ECM) proteins in a CXCR2-dependent manner. CT-26 cells expressed the αV, β1 and β3 integrin subunits and immunoneutralization of αV abolished CXCL2-triggered adhesion of CT-26 to vitronectin, fibronectin and fibrinogen. Finally, inhibition of the αV integrin significantly attenuated the number of carcinomatosis nodules by 69% in laparotomized mice. These results were validated by use of the human colon cancer cell line HT-29 in vitro. Our data show that colon cancer cell adhesion and growth on peritoneal wound sites is mediated by a CXCL2-CXCR2 signaling axis and αV integrin-dependent adhesion to ECM proteins. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
653 | a Chemokines | |
653 | a Chemotaxis | |
653 | a Integrins | |
653 | a Peritoneal carcinomatosis | |
653 | a Metastasis | |
700 | 1 | a Algethami, Naderu Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)na1052al |
700 | 1 | a Al-Haidari, Amr Au Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)med-ama |
700 | 1 | a Algaber, Anwaru Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)an6321al |
700 | 1 | a Syk, Ingvaru Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)kir-isy |
700 | 1 | a Rahman, Milladuru Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups4 aut0 (Swepub:lu)med-mdr |
700 | 1 | a Thorlacius, Henriku Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Kirurgi,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Department of Clinical Sciences, Malmö,Faculty of Medicine,Surgery,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments4 aut0 (Swepub:lu)kir-hth |
710 | 2 | a Kirurgib Forskargrupper vid Lunds universitet4 org |
773 | 0 | t Clinical and Experimental Metastasisd : Springer Science and Business Media LLCg 38:4, s. 401-410q 38:4<401-410x 1573-7276x 0262-0898 |
856 | 4 | u http://dx.doi.org/10.1007/s10585-021-10103-0x freey FULLTEXT |
856 | 4 | u https://link.springer.com/content/pdf/10.1007/s10585-021-10103-0.pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/42d1fc68-d230-4713-84dd-d57b243183e2 |
856 | 4 8 | u https://doi.org/10.1007/s10585-021-10103-0 |
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