L773:0021 9673
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 05393naa a2200481 4500 | |
| 001 | oai:lup.lub.lu.se:d714c6d3-8ff7-4a90-8042-b36af34276f4 | |
| 003 | SwePub | |
| 008 | 160401s2002 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/3242222 URI |
| 024 | 7 | a https://doi.org/10.1016/S0021-9673(02)01114-72 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Arvidsson, Päru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-par |
| 245 | 1 0 | a Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns |
| 264 | 1 | c 2002 |
| 520 | a Continuous supermacroporous chromatographic columns with anion-exchange ligands [2-(dimethylanlino)ethyl group] and immobilized metal affinity (IMA) ligands (Cu2+-loaded iminodiacetic acid) have been developed allowing binding of Escherichia coli cells and the elution of bound cells with high recoveries. These poly(acrylamide)-based continuous supermacroporous columns have been produced by radical co-polymerization of monomers in aqueous solution frozen inside a column (cryo-polymerization). After thawing, the column contains a continuous matrix (so-called cryogel) with interconnected pores of 10-100 mum in size. The large pore size of the matrix makes it possible for E. coli cells to pass unhindered through a plain column containing no ligands. E. coli cells bound to an ion-exchange column at low ionic strength were eluted with 70-80% recovery at NaCl concentrations of 0.35-0.40 M, while cells bound to an IMA-column were eluted with around 80% recovery using either 10 mM imidazole or 20 mM EDTA solutions, respectively. The cells maintain their viability after the binding/elution procedure. These preliminary results indicate that microbial cells can be handled in a chromatographic mode using supermacroporous continuous columns. These columns are easy to manufacture from cheap and readily available starting materials, which make the columns suitable for single-time use. (C) 2002 Published by Elsevier Science B.V. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a metal affinity chromatography | |
| 653 | a immobilized | |
| 653 | a ion-exchange chromatography | |
| 653 | a cells | |
| 653 | a LC | |
| 653 | a stationary phases | |
| 653 | a bacteria | |
| 653 | a proteins | |
| 700 | 1 | a Plieva, Fatimau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-fpl |
| 700 | 1 | a Savina, Irinau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-is0 |
| 700 | 1 | a Lozinsky, VI4 aut |
| 700 | 1 | a Fexby, Sarau Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)tbk-sfe |
| 700 | 1 | a Bülow, Leifu Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)tbk-lbu |
| 700 | 1 | a Galaev, Igoru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-iga |
| 700 | 1 | a Mattiasson, Bou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-bma |
| 710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
| 773 | 0 | t Journal of Chromatography Ag 977:1, s. 27-38q 977:1<27-38x 0021-9673 |
| 856 | 4 | u http://dx.doi.org/10.1016/S0021-9673(02)01114-7y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/324222 |
| 856 | 4 8 | u https://doi.org/10.1016/S0021-9673(02)01114-7 |
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