Transcriptional activity and developmental expression of liver X receptor (lxr) in zebrafish

QC 20230411
Mammalian liver-X-receptors (LXRs) are transcription factors activated by oxysterols. They play an essential role in lipid and glucose metabolism. We have cloned the open reading frame of zebrafish lxr and describe its genomic organization. Zebrafish lxr encodes a 50-kDa protein with high sequence similarity to mammalian LXR alpha. In transfection assays, the encoded protein showed transcriptional activity in response to LXR-ligands. Treatment of adult zebrafish with the synthetic LXR ligand, GW3965, induced expression of genes involved in hepatic cholesterol and lipid pathways. Using qPCR and in situ hybridization, we found ubiquitous expression of lxr mRNA during the first 24 hr of development, followed by more restricted expression, particularly to the liver at 3dpf and the liver and intestine at 4dpf. In adult fish, all examined organs expressed lxr. In addition to a metabolic role of lxr, the temporal expression pattern suggests a developmental role in, e.g., the liver and CNS.

Lauter, GilbertKarolinska Institutet,Södertörns högskola,Institutionen för livsvetenskaper(Swepub:sh)shgtlr03 (author)
Hauptmann, GiselbertKarolinska Institutet,Södertörns högskola,Institutionen för livsvetenskaper(Swepub:sh)SHGTHN02 (author)
Mode, Agneta (author)
Gustafsson, Jan-Ake (author)
Karolinska InstitutetInstitutionen för livsvetenskaper (creator_code:org_t)

By the author/editor: Archer, Amena; Lauter, Gilbert; Hauptmann, Gisel ...; Mode, Agneta; Gustafsson, Jan- ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Developmental Bi ...

By the university: Södertörn University; Royal Institute of Technology; Karolinska Institutet

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