JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression

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Fältnamn	Indikatorer	Metadata
000		02498naa a2200313 4500
001		oai:prod.swepub.kib.ki.se:132861126
003		SwePub
008		240701s2016 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1328611262 URI
024	7	a https://doi.org/10.1155/2016/13127052 DOI
040		a (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Prause, M4 aut
245	1 0	a JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression
264	1	b Hindawi Limited,c 2016
520		a The relative contributions of the JNK subtypes in inflammatoryβ-cell failure and apoptosis are unclear. The JNK protein family consists of JNK1, JNK2, and JNK3 subtypes, encompassing many different isoforms. INS-1 cells express JNK1α1, JNK1α2, JNK1β1, JNK1β2, JNK2α1, JNK2α2, JNK3α1, and JNK3α2 mRNA isoform transcripts translating into 46 and 54 kDa isoform JNK proteins. Utilizing Lentiviral mediated expression of shRNAs against JNK1, JNK2, or JNK3 in insulin-producing INS-1 cells, we investigated the role of individual JNK subtypes in IL-1β-inducedβ-cell apoptosis. JNK1 knockdown prevented IL-1β-induced INS-1 cell apoptosis associated with decreased 46 kDa isoform JNK protein phosphorylation and attenuated Myc expression. Transient knockdown of Myc also prevented IL-1β-induced apoptosis as well as caspase 3 cleavage. JNK2 shRNA potentiated IL-1β-induced apoptosis and caspase 3 cleavage, whereas JNK3 shRNA did not affect IL-1β-inducedβ-cell death compared to nonsense shRNA expressing INS-1 cells. In conclusion, JNK1 mediates INS-1 cell death associated with increased Myc expression. These findings underline the importance of differentiated targeting of JNK subtypes in the development of inflammatoryβ-cell failure and destruction.
700	1	a Mayer, CM4 aut
700	1	a Brorsson, C4 aut
700	1	a Frederiksen, KS4 aut
700	1	a Billestrup, N4 aut
700	1	a Storling, J4 aut
700	1	a Mandrup-Poulsen, T4 aut
773	0	t Journal of diabetes researchd : Hindawi Limitedg 2016, s. 1312705-q 2016<1312705-x 2314-6753x 2314-6745
856	4	u http://downloads.hindawi.com/journals/jdr/2016/1312705.pdf
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:132861126
856	4 8	u https://doi.org/10.1155/2016/1312705

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Av författaren/redakt...: Prause, M; Mayer, CM; Brorsson, C; Frederiksen, KS; Billestrup, N; Storling, J; visa fler...; Mandrup-Poulsen, ...; visa färre...

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Av lärosätet: Karolinska Institutet

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