WFRF:(Andrew R.)
Search: WFRF:(Andrew R.) > (1991-1994) > Molecular character...
- 3 of 4
- Previous record
- Next record
- To hitlist
Molecular characterization of a plasmid-borne (pGT633) erythromycin resistance determinant (ermGT) from lactobacillus reuteri 100-63
- Tannock, Gerald W. (author)
-
- Luchansky, John B. (author)
- University of Wisconsin-Madison
-
- Miller, Lynette (author)
- North Carolina State University
- show more...
- show less...
- (creator_code:org_t)
- Elsevier BV, 1994
- 1994
- English 12 s.
- In: Plasmid. - : Elsevier BV. - 0147-619X. ; 31:1, s. 60-71
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Lactobacillus reuteri 100-63 contains a 9.8 kb plasmid (pGT633) encoding resistance to the macrolide-lincosamide-streptogramin (MLS) type B antibiotics. The restriction endonucleases AccI, BamHI, Bcl I, EcoRI, EcoRV, HhaI, HindII, Hpa I, KpnI, SalI, and SspI were employed to establish a physical map of pGT633. Next, genetic transfer experiments were conducted to establish the host range of pGT633. The results revealed that pGT633 replicated autonomously and encoded constitutive resistance to erthromycin in a variety of bacterial hosts, including Bacillus subtilis BD170, Streptococcus sanguis DL1, Staphylococcus aureus RN4220, and Enterococcus faecalis 19433, as well as several Lactobacillus spp. A 2.2 kb BamHI fragment of pGT633 containing the genetic determinant (ermGT) encoding resistance to erythromycin was cloned into Escherichia coli. Determination of the nucleotide sequence of ermGT revealed a methylase gene 731 bp in length that was highly related (ca. 81% nucleotide and ca. 78% amino acid identity) to the ermC gene from S. aureus plasmid pE194. The leader sequences of ermGT and ermC were identical except for a single base change at nt 51.
Publication and Content Type
- art (subject category)
- ref (subject category)
- 3 of 4
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Tannock, Gerald ...
- Luchansky, John ...
- Miller, Lynette
- Connell, Hugh
- Thode-Andersen, ...
- Mercer, Andrew A ...
- show more...
- Klaenhammer, Tod ...
- show less...
- Articles in the publication
- Plasmid
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
