Gemini surfactants: New synthetic vectors for gene transfection

Kirby, A J (author)

Camilleri, P (author)

Engberts, J (author)

Feiters, M. C. (author)

Nolte, R J M (author)

Söderman, Olle (author): Lund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH

Bergsma, M (author)

Bell, P C (author)

Fielden, M L (author)

Rodriguez, C L G (author)

Guedat, P (author)

Kremer, A (author)

McGregor, C (author)

Perrin, C (author)

Ronsin, G (author)

van Eijk, Marcel C P (author)

(creator_code:org_t)

Wiley, 2003
2003
English.
In: Angewandte Chemie International Edition in English. - : Wiley. - 0570-0833 .- 1433-7851 .- 1521-3773. ; 42:13, s. 1448-1457

Related links:: http://dx.doi.org/10...; show more...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Abstract Subject headings

The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.

By the author/editor: Kirby, A J; Camilleri, P; Engberts, J; Feiters, M. C.; Nolte, R J M; Söderman, Olle; show more...; Bergsma, M; Bell, P C; Fielden, M L; Rodriguez, C L G; Guedat, P; Kremer, A; McGregor, C; Perrin, C; Ronsin, G; van Eijk, Marcel ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

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