Sökning: WFRF:(Babu Moparthi Satish) >
Transient conformat...
Transient conformational remodeling of folding proteins by GroES - Individually and in concert with GroEL
-
- Babu Moparthi, Satish (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten,Institut Fresnel, CNRS UMR 7249, Aix-Marseille Université, Marseille, France
-
- Sjölander, Daniel (författare)
- Linköpings universitet,Kemi,Tekniska högskolan
-
- Villebeck, Laila (författare)
- Linköpings universitet,Molekylär Bioteknik,Tekniska högskolan
-
visa fler...
-
- Jonsson, Bengt-Harald (författare)
- Linköpings universitet,Kemi,Tekniska högskolan
-
- Hammarström, Per (författare)
- Linköpings universitet,Kemi,Tekniska högskolan
-
- Carlsson, Uno (författare)
- Linköpings universitet,Kemi,Tekniska högskolan
-
visa färre...
-
(creator_code:org_t)
- 2013-10-05
- 2014
- Engelska.
-
Ingår i: Journal of chemical biology. - : Springer Berlin/Heidelberg. - 1864-6158 .- 1864-6166. ; 7:1, s. 1-15
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://link.springe...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The commonly accepted dogma of the bacterial GroE chaperonin system entails protein folding mediated by cycles of several ATP-dependent sequential steps where GroEL interacts with the folding client protein. In contrast, we herein report GroES-mediated dynamic remodeling (expansion and compression) of two different protein substrates during folding: the endogenous substrate MreB and carbonic anhydrase (HCAII), a well-characterized protein folding model. GroES was also found to influence GroEL binding induced unfolding and compression of the client protein underlining the synergistic activity of both chaperonins, even in the absence of ATP. This previously unidentified activity by GroES should have important implications for understanding the chaperonin mechanism and cellular stress response. Our findings necessitate a revision of the GroEL/ES mechanism.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Carbonic anhydrase; Chaperone; FRET; Molten globule; MreB; Protein folding
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
Hitta via bibliotek
Till lärosätets databas