Bioactive compounds isolated from submerged fermentations of the Chilean fungus Stereum rameale

• Liquid fermentations of the fungus Stereum rameale (No 2511) yielded extracts with antibacterial -activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 mu g/mL), Bacillus subtilis (10 mu g/mL) and Staphylococcus aureus (100 mu g/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 mu g/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 mu g/mL, respectively.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

antibacterial activity

MIC

MS-3

Stereum rameale

vibralactone

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)