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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005715naa a2200541 4500
001oai:DiVA.org:umu-200031
003SwePub
008221010s2016 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-2000312 URI
024a https://doi.org/10.1093/jxb/erw3662 DOI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Li, Luu Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
2451 0a Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana
264 c 2016-10-06
264 1b Oxford University Press,c 2016
338 a electronic2 rdacarrier
520 a In plant cells, mitochondria are major providers of energy and building blocks for growth and development as well as abiotic and biotic stress responses. They are encircled by two lipid membranes containing proteins that control mitochondrial function through the import of macromolecules and metabolites. Characterization of a novel β-barrel protein, OUTER MEMBRANE PROTEIN 47 (OM47), unique to the green lineage and related to the voltage-dependent anion channel (VDAC) protein family, showed that OM47 can complement a VDAC mutant in yeast. Mutation of OM47 in Arabidopsis thaliana by T-DNA insertion had no effect on the import of proteins, such as the β-barrel proteins translocase of the outer membrane 40 (TOM40) or sorting and assembly machinery 50 (SAM50), into mitochondria. Molecular and physiological analyses revealed a delay in chlorophyll breakdown, higher levels of starch, and a delay in the induction of senescence marker genes in the mutant lines. While there was a reduction of >90% in OM47 protein in mitochondria isolated from 3-week-old om47 mutants, in mitochondria isolated from 8-week-old plants OM47 levels were similar to that of the wild type. This recovery was achieved by an up-regulation of OM47 transcript abundance in the mutants. Combined, these results highlight a role in leaf senescence for this plant-specific β-barrel protein, probably mediating the recovery and recycling of chloroplast breakdown products by transporting metabolic intermediates into and out of mitochondria.
650 7a NATURVETENSKAPx Biologix Botanik0 (SwePub)106072 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Botany0 (SwePub)106072 hsv//eng
653 a Arabidopsis
653 a At3g27930
653 a membrane transport
653 a mitochondria
653 a OM47
653 a senescence
653 a VDAC
653 a β-barrel protein
700a Kubiszewski-Jakubiak, Szymonu Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, WA, Crawley, Australia4 aut
700a Radomiljac, Jordanu Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
700a Wang, Yanu Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
700a Law, Simon R.u Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)4 aut0 (Swepub:umu)sila0026
700a Keech, Olivieru Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)4 aut0 (Swepub:umu)olke0001
700a Narsai, Reenau Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
700a Berkowitz, Oliveru Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
700a Duncan, Owenu Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, WA, Crawley, Australia4 aut
700a Murcha, Monika W.u Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, WA, Crawley, Australia4 aut
700a Whelan, Jamesu Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australia4 aut
710a Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, VIC, Bundoora, Australiab Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, WA, Crawley, Australia4 org
773t Journal of Experimental Botanyd : Oxford University Pressg 67:21, s. 6061-6075q 67:21<6061-6075x 0022-0957x 1460-2431
856u https://doi.org/10.1093/jxb/erw366y Fulltext
856u https://umu.diva-portal.org/smash/get/diva2:1702046/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856u https://academic.oup.com/jxb/article-pdf/67/21/6061/17142919/erw366.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-200031
8564 8u https://doi.org/10.1093/jxb/erw366

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