WFRF:(Cook N. J.)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02935naa a2200385 4500 | |
| 001 | oai:lup.lub.lu.se:d9a7f958-97c2-415b-b0d7-1f447cd75932 | |
| 003 | SwePub | |
| 008 | 160401s2003 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/1328202 URI |
| 024 | 7 | a https://doi.org/10.1016/S0168-1605(02)00322-72 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Malorny, B4 aut |
| 245 | 1 0 | a Standardization of diagnostic PCR for the detection of foodborne pathogens. |
| 264 | 1 | c 2003 |
| 520 | a In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections as well as for the analysis of microorganisms in food samples. However, despite its potential, PCR has neither gained wide acceptance in routine diagnostics nor been widely incorporated in standardized methods. Lack of validation and standard protocols, as well as variable quality of reagents and equipment, influence the efficient dissemination of PCR methodology from expert research laboratories to end-user laboratories. Moreover, the food industry understandably requires and expects officially approved standards. Recognizing this, in 1999, the European Commission approved the research project, FOOD-PCR (http://www.PCR.dk), which aims to validate and standardize the use of diagnostic PCR for the detection of pathogenic bacteria in foods. The present review focuses on the harmonization procedure and standardization criteria for detection of foodborne pathogens by PCR. The progress of standardization so far and future perspectives of diagnostic PCR are discussed. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
| 653 | a PCR | |
| 653 | a Harmonization and standardization | |
| 653 | a Detection | |
| 653 | a Foodborne pathogens | |
| 700 | 1 | a Tassios, P T4 aut |
| 700 | 1 | a Rådström, Peteru Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)tmb-pra |
| 700 | 1 | a Cook, N4 aut |
| 700 | 1 | a Wagner, M4 aut |
| 700 | 1 | a Hoorfar, J4 aut |
| 710 | 2 | a Teknisk mikrobiologib Centrum för tillämpade biovetenskaper4 org |
| 773 | 0 | t International Journal of Food Microbiologyg 83:1, s. 39-48q 83:1<39-48x 0168-1605 |
| 856 | 4 | u http://dx.doi.org/10.1016/S0168-1605(02)00322-7y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/132820 |
| 856 | 4 8 | u https://doi.org/10.1016/S0168-1605(02)00322-7 |
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