Sökning: WFRF:(Holmberg Bjorn) > High throughput gen...
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000 | 05904naa a2200709 4500 | |
001 | oai:DiVA.org:kth-278779 | |
003 | SwePub | |
008 | 200729s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2787792 URI |
024 | 7 | a https://doi.org/10.1016/j.nbt.2020.05.0022 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Tegel, Hannau KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1nou8yx |
245 | 1 0 | a High throughput generation of a resource of the human secretome in mammalian cells |
264 | 1 | b Elsevier BV,c 2020 |
338 | a print2 rdacarrier | |
500 | a QC 20200729 | |
520 | a The proteins secreted by human tissues and blood cells, the secretome, are important both for the basic understanding of human biology and for identification of potential targets for future diagnosis and therapy. Here, a high-throughput mammalian cell factory is presented that was established to create a resource of recombinant full-length proteins covering the majority of those annotated as 'secreted' in humans. The full-length DNA sequences of each of the predicted secreted proteins were generated by gene synthesis, the constructs were transfected into Chinese hamster ovary (CHO) cells and the recombinant proteins were produced, purified and analyzed. Almost 1,300 proteins were successfully generated and proteins predicted to be secreted into the blood were produced with a success rate of 65%, while the success rates for the other categories of secreted proteins were somewhat lower giving an overall one-pass success rate of ca. 58%. The proteins were used to generate targeted proteomics assays and several of the proteins were shown to be active in a phenotypic assay involving pancreatic beta-cell dedifferentiation. Many of the proteins that failed during production in CHO cells could be rescued in human embryonic kidney (HEK 293) cells suggesting that a cell factory of human origin can be an attractive alternative for production in mammalian cells. In conclusion, a high-throughput protein production and purification system has been successfully established to create a unique resource of the human secretome. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a secreted proteins | |
653 | a protein production | |
653 | a protein puri fication | |
653 | a high-throughput | |
700 | 1 | a Dannemeyer, Melanieu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1u0d39z |
700 | 1 | a Kanje, Sara,d 1986-u KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1t204cn |
700 | 1 | a Sivertsson, Åsau KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u195d5b0 |
700 | 1 | a Berling, Annau KTH,Proteinteknologi4 aut0 (Swepub:kth)u18q4b67 |
700 | 1 | a Svensson, Anne-Sophieu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1t0mckt |
700 | 1 | a Hober, Andreasu KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u15bkxsl |
700 | 1 | a Enstedt, Henricu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1svlatx |
700 | 1 | a Volk, Anna-Luisau KTH,Proteinteknologi4 aut0 (Swepub:kth)u1j049o2 |
700 | 1 | a Lundqvist, Magnusu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1hfekeg |
700 | 1 | a Moradi, Mona4 aut |
700 | 1 | a Afshari, Delaramu KTH,Proteinteknologi4 aut0 (Swepub:kth)u18nn4qt |
700 | 1 | a Ekblad, Siriu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u13gulih |
700 | 1 | a Xu, LanLanu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1qu1kuf |
700 | 1 | a Vestin, Malinu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1qp5jk2 |
700 | 1 | a Bidad, Faranaku KTH,Proteinteknologi4 aut0 (Swepub:kth)u13gbknj |
700 | 1 | a Schiavone, Lovisa Holmbergu AstraZeneca, R&D, Discovery Biol, Discovery Sci, Gothenburg, Sweden.4 aut |
700 | 1 | a Davies, Ricku AstraZeneca, R&D, Discovery Biol, Discovery Sci, Cambridge, England.4 aut |
700 | 1 | a Mayr, Lorenz M.u Syncona Investment Ltd, London, England.;Swiss Fed Inst Technol, B BIOL, Mol Hlth Sci, Zurich, Switzerland.4 aut |
700 | 1 | a Knight, Sineadu AstraZeneca, R&D, Discovery Biol, Discovery Sci, Cambridge, England.4 aut |
700 | 1 | a Gopel, Sven O.u AstraZeneca, BioPharmaceut R&D, Biosci Metab Res & Early Dev Cardiovasc Renal & M, Gothenburg, Sweden.4 aut |
700 | 1 | a Voldborg, Bjorn G.u Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Lyngby, Denmark.4 aut |
700 | 1 | a Edfors, Fredriku KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u149ml0e |
700 | 1 | a Forsström, Björnu KTH,Science for Life Laboratory, SciLifeLab,Proteinteknologi4 aut0 (Swepub:kth)u11i5nju |
700 | 1 | a von Feilitzen, Kalleu KTH,Proteinteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dbmi38 |
700 | 1 | a Zwahlen, Martinu KTH,Science for Life Laboratory, SciLifeLab,Proteinteknologi4 aut0 (Swepub:kth)u1fcjpxe |
700 | 1 | a Rockberg, Johanu KTH,Proteinteknologi4 aut0 (Swepub:kth)u130jo0a |
700 | 1 | a Takanen, Jenny Ottossonu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1lthxtb |
700 | 1 | a Uhlén, Mathiasu KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dulvmw |
700 | 1 | a Hober, Sophia,d 1965-u KTH,Proteinteknologi4 aut0 (Swepub:kth)u11qqzc1 |
710 | 2 | a KTHb Proteinvetenskap4 org |
773 | 0 | t New Biotechnologyd : Elsevier BVg 58, s. 45-54q 58<45-54x 1871-6784x 1876-4347 |
856 | 4 | u https://doi.org/10.1016/j.nbt.2020.05.002y Fulltext |
856 | 4 | u https://doi.org/10.1016/j.nbt.2020.05.002 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278779 |
856 | 4 8 | u https://doi.org/10.1016/j.nbt.2020.05.002 |
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