Search: WFRF:(Karlsson Hedestam Gunilla)
> (2020-2023) >
Vaccination of SARS...
Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages
-
- Chernyshev, Mark (author)
- Karolinska Institutet
-
- Sakharkar, Mrunal (author)
- NH, United States
-
- Connor, Ruth I. (author)
- Department of Pediatrics, Dartmouth-Hitchcock Medical Center, NH, United States
-
show more...
-
- Dugan, Haley L. (author)
- NH, United States
-
- Sheward, Daniel J. (author)
- Karolinska Institutet
-
- Rappazzo, C.G. (author)
- NH, United States
-
- Stålmarck, Aron (author)
- Karolinska Institutet
-
- Forsell, Mattias N. E. (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Wright, Peter F. (author)
- Department of Pediatrics, Dartmouth-Hitchcock Medical Center, NH, United States
-
- Corcoran, Martin (author)
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
-
- Murrell, Ben (author)
- Karolinska Institutet
-
- Walker, Laura M. (author)
- NH, United States, MA, Waltham, United States
-
- Karlsson Hedestam, Gunilla B. (author)
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
-
show less...
-
(creator_code:org_t)
- Springer Nature, 2023
- 2023
- English.
-
In: Nature Communications. - : Springer Nature. - 2041-1723. ; 14:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://umu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Vaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolate 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with the index variant of SARS-CoV-2 and later boosted with mRNA-1273. We characterize mAb genetic features by sequence assignments to the donors' personal immunoglobulin genotypes and assess antibody neutralizing activities against index SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells are efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Chernyshev, Mark
-
Sakharkar, Mruna ...
-
Connor, Ruth I.
-
Dugan, Haley L.
-
Sheward, Daniel ...
-
Rappazzo, C.G.
-
show more...
-
Stålmarck, Aron
-
Forsell, Mattias ...
-
Wright, Peter F.
-
Corcoran, Martin
-
Murrell, Ben
-
Walker, Laura M.
-
Karlsson Hedesta ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
- Articles in the publication
-
Nature Communica ...
- By the university
-
Umeå University
-
Karolinska Institutet