Sökning: WFRF:(Lind ) > Impact of ocean aci...
Fältnamn | Indikatorer | Metadata |
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000 | 06367naa a2200781 4500 | |
001 | oai:DiVA.org:hkr-15677 | |
003 | SwePub | |
008 | 160811s2016 | |||||||||||000 ||eng| | |
009 | oai:gup.ub.gu.se/235145 | |
009 | oai:DiVA.org:uu-284520 | |
024 | 7 | a urn:nbn:se:hkr:diva-156772 urn |
024 | 7 | a https://doi.org/10.1016/j.fsi.2016.04.0072 DOI |
024 | 7 | a https://gup.ub.gu.se/publication/2351452 URI |
024 | 7 | a urn:nbn:se:uu:diva-2845202 urn |
040 | a (SwePub)hkrd (SwePub)gu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Hernroth, Bodil,d 1951-u Högskolan Kristianstad,Avdelningen för Naturvetenskap,Forskningsmiljön Man & Biosphere Health (MABH),Royal Swedish Acad Sci, Sven Loven Ctr Marine Sci, Kristineberg 566, SE-45178 Fiskebackskil, Sweden;Kristianstad Univ, Dept Nat Sci, SE-29188 Kristianstad, Sweden4 aut0 (Swepub:hkr)heb |
245 | 1 0 | a Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
264 | 1 | b Elsevier,c 2016 |
338 | a print2 rdacarrier | |
520 | a Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from ∼8.1 to ∼7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by ∼65–90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5–3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens. | |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologiska vetenskaperx Immunologi0 (SwePub)106052 hsv//swe |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
650 | 7 | a AGRICULTURAL SCIENCESx Agricultural Science, Forestry and Fisheriesx Fish and Aquacultural Science0 (SwePub)401072 hsv//eng |
650 | 7 | a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiskex Fisk- och akvakulturforskning0 (SwePub)401072 hsv//swe |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiske0 (SwePub)4012 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
650 | 7 | a AGRICULTURAL SCIENCESx Agriculture, Forestry and Fisheries0 (SwePub)4012 hsv//eng |
653 | a Ocean acidification | |
653 | a Mytilus edulis | |
653 | a Antimicrobial peptide | |
653 | a Gill tissue | |
653 | a Vibrio | |
653 | a LPS | |
653 | a Proteome analysis | |
653 | a LC-Orbitrap MS/MS analysis | |
653 | a Ocean acidification; Mytilus edulis; Antimicrobial peptide; Gill tissue; Vibrio; LPS; Proteome analysis; LC-Orbitrap MS/MS analysis | |
700 | 1 | a Baden, S.u University of Gothenburg4 aut |
700 | 1 | a Tassidis, Helenau Högskolan Kristianstad,Avdelningen för Naturvetenskap,Forskningsmiljön Man & Biosphere Health (MABH),Kristianstad Univ, Dept Nat Sci, SE-29188 Kristianstad, Sweden4 aut0 (Swepub:hkr)tashel |
700 | 1 | a Hörnaeus, K.u Uppsala University4 aut |
700 | 1 | a Guillemant, J.u Uppsala University4 aut |
700 | 1 | a Bergström Lind, S.u Uppsala University4 aut |
700 | 1 | a Bergquist, J.u Uppsala University4 aut |
700 | 1 | a Lind, SB4 aut |
700 | 1 | a Baden, Susanne P.,d 1952u Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences4 aut0 (Swepub:gu)xbadsu |
700 | 1 | a Bergqvist, Jonas4 aut |
700 | 1 | a Tassidis, H.4 aut |
700 | 1 | a Baden, Susanneu Univ Gothenburg, Dept Biol & Environm Sci, Kristineberg 566, SE-45178 Fiskebackskil, Sweden4 aut |
700 | 1 | a Guillemant, Julieu Uppsala universitet,Analytisk kemi,Lind4 aut |
700 | 1 | a Bergström Lind, Sarau Uppsala universitet,Analytisk kemi,Lind4 aut0 (Swepub:uu)saber676 |
700 | 1 | a Bergquist, Jonasu Uppsala universitet,Analytisk kemi,Bergquist4 aut0 (Swepub:uu)jbe25356 |
700 | 1 | a Hörnaeus, Katarinau Uppsala universitet,Analytisk kemi,Ramström4 aut0 (Swepub:uu)katho286 |
710 | 2 | a Högskolan Kristianstadb Avdelningen för Naturvetenskap4 org |
710 | 2 | a Gothenburg University |
710 | 2 | a Göteborgs universitet |
773 | 0 | t Fish and Shellfish Immunologyd : Elsevierg 55, s. 452-459q 55<452-459x 1050-4648x 1095-9947 |
856 | 4 8 | u http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-15677x lärosäteslänky Till lärosätets (hkr) databas |
856 | 4 8 | u https://doi.org/10.1016/j.fsi.2016.04.007 |
856 | 4 8 | u https://gup.ub.gu.se/publication/235145 |
856 | 4 8 | u http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-284520x lärosäteslänky Till lärosätets (uu) databas |
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