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FältnamnIndikatorerMetadata
00006367naa a2200781 4500
001oai:DiVA.org:hkr-15677
003SwePub
008160811s2016 | |||||||||||000 ||eng|
009oai:gup.ub.gu.se/235145
009oai:DiVA.org:uu-284520
024a urn:nbn:se:hkr:diva-156772 urn
024a https://doi.org/10.1016/j.fsi.2016.04.0072 DOI
024a https://gup.ub.gu.se/publication/2351452 URI
024a urn:nbn:se:uu:diva-2845202 urn
040 a (SwePub)hkrd (SwePub)gu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Hernroth, Bodil,d 1951-u Högskolan Kristianstad,Avdelningen för Naturvetenskap,Forskningsmiljön Man & Biosphere Health (MABH),Royal Swedish Acad Sci, Sven Loven Ctr Marine Sci, Kristineberg 566, SE-45178 Fiskebackskil, Sweden;Kristianstad Univ, Dept Nat Sci, SE-29188 Kristianstad, Sweden4 aut0 (Swepub:hkr)heb
2451 0a Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)
264 1b Elsevier,c 2016
338 a print2 rdacarrier
520 a Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from ∼8.1 to ∼7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by ∼65–90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5–3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens.
650 7a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng
650 7a NATURVETENSKAPx Biologiska vetenskaperx Immunologi0 (SwePub)106052 hsv//swe
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
650 7a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
650 7a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650 7a AGRICULTURAL SCIENCESx Agricultural Science, Forestry and Fisheriesx Fish and Aquacultural Science0 (SwePub)401072 hsv//eng
650 7a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiskex Fisk- och akvakulturforskning0 (SwePub)401072 hsv//swe
650 7a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650 7a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiske0 (SwePub)4012 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
650 7a AGRICULTURAL SCIENCESx Agriculture, Forestry and Fisheries0 (SwePub)4012 hsv//eng
653 a Ocean acidification
653 a Mytilus edulis
653 a Antimicrobial peptide
653 a Gill tissue
653 a Vibrio
653 a LPS
653 a Proteome analysis
653 a LC-Orbitrap MS/MS analysis
653 a Ocean acidification; Mytilus edulis; Antimicrobial peptide; Gill tissue; Vibrio; LPS; Proteome analysis; LC-Orbitrap MS/MS analysis
700a Baden, S.u University of Gothenburg4 aut
700a Tassidis, Helenau Högskolan Kristianstad,Avdelningen för Naturvetenskap,Forskningsmiljön Man & Biosphere Health (MABH),Kristianstad Univ, Dept Nat Sci, SE-29188 Kristianstad, Sweden4 aut0 (Swepub:hkr)tashel
700a Hörnaeus, K.u Uppsala University4 aut
700a Guillemant, J.u Uppsala University4 aut
700a Bergström Lind, S.u Uppsala University4 aut
700a Bergquist, J.u Uppsala University4 aut
700a Lind, SB4 aut
700a Baden, Susanne P.,d 1952u Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences4 aut0 (Swepub:gu)xbadsu
700a Bergqvist, Jonas4 aut
700a Tassidis, H.4 aut
700a Baden, Susanneu Univ Gothenburg, Dept Biol & Environm Sci, Kristineberg 566, SE-45178 Fiskebackskil, Sweden4 aut
700a Guillemant, Julieu Uppsala universitet,Analytisk kemi,Lind4 aut
700a Bergström Lind, Sarau Uppsala universitet,Analytisk kemi,Lind4 aut0 (Swepub:uu)saber676
700a Bergquist, Jonasu Uppsala universitet,Analytisk kemi,Bergquist4 aut0 (Swepub:uu)jbe25356
700a Hörnaeus, Katarinau Uppsala universitet,Analytisk kemi,Ramström4 aut0 (Swepub:uu)katho286
710a Högskolan Kristianstadb Avdelningen för Naturvetenskap4 org
710a Gothenburg University
710a Göteborgs universitet
773t Fish and Shellfish Immunologyd : Elsevierg 55, s. 452-459q 55<452-459x 1050-4648x 1095-9947
8564 8u http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-15677x lärosäteslänky Till lärosätets (hkr) databas
8564 8u https://doi.org/10.1016/j.fsi.2016.04.007
8564 8u https://gup.ub.gu.se/publication/235145
8564 8u http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-284520x lärosäteslänky Till lärosätets (uu) databas

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