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Validation of an MP...
Validation of an MPC polymer coating to reduce surface-induced cascade system activation in whole blood in in vitroand in vivo models
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- Asif, Sana, M.D, PhD student (author)
- Uppsala universitet,Klinisk immunologi
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Asawa, Kenta (author)
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Yuuki, Inoue (author)
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Kazuhiko, Ishihara2 (author)
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- Lindell, Björn (author)
- Uppsala universitet,Käkkirurgi
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- Holmgren, Robin (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Nilsson, Bo (author)
- Uppsala universitet,Klinisk immunologi
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Ryden, Anneli (author)
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Wearn, Marinne Jensen (author)
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- Teramura, Yuji (author)
- Uppsala universitet,Klinisk immunologi
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- Nilsson Ekdahl, Kristina (author)
- Uppsala universitet,Klinisk immunologi,Fasta tillståndets fysik
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- ABSTRACTBackground: Artificial surfaces that come into contact with blood (e.g., when used in various forms of biomedical device) induce an immediate activation of the cascade systems of the blood, the coagulation and complement systems. These reactions may lead to a thrombotic and/or inflammatory response that can eventually cause damage to the biomaterial or the patient, or to both. Multiple strategies to dampen these reactions have been employed, with heparin conjugation to the material surface being the most successfulthus far. Another approach to improving hemocompatibility is to use 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer coatings.Experimental: In the present study, we evaluated the effectiveness of MPC polymer coating and compared it to a commercially available heparin coating in various in vitromodels using fresh human blood with the aim to replace the costly heparin-coated equipment with the more economic MPC. We then investigated the stability of the various coatings in human plasma in vitrofor 2 weeks. Finally, we inserted MPC polymer-coated catheters into the external jugular vein of pigs and monitored the catheters’ antithrombotic properties for 4 days.Results: 1) There was no significant activation of platelets and of the coagulation and complement systems on the MPC polymer-coated or the commercially available heparin surface. 2) Both coats were superior in hemocompatibility to non-coated matrix surfaces. 3) The protective effect of the MPC polymer coat did not decline after incubation in plasma for up to 2 weeks. 4) With MPC polymer-coated catheters, it was possible to easily draw blood from experimental animals for 4 days, in contrast to the case for heparin-flushed commercially available non-coated catheters, in which substantial clotting was seen.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Publication and Content Type
- vet (subject category)
- ovr (subject category)
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- By the author/editor
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Asif, Sana, M.D, ...
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Asawa, Kenta
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Yuuki, Inoue
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Kazuhiko, Ishiha ...
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Lindell, Björn
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Holmgren, Robin
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show more...
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Nilsson, Bo
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Ryden, Anneli
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Wearn, Marinne J ...
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Teramura, Yuji
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Nilsson Ekdahl, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Other Clinical M ...
- By the university
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Uppsala University