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Expression of a Dro...
Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene
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Tzafestas, Kyriakos (author)
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Razalan, Maria M. (author)
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Gyulev, Ivan (author)
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- Mazari, Aslam M. A. (author)
- Stockholms universitet,Institutionen för neurokemi
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- Mannervik, Bengt (author)
- Stockholms universitet,Institutionen för neurokemi
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Rylott, Elizabeth L. (author)
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Bruce, Neil C. (author)
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(creator_code:org_t)
- 2016-12-06
- 2017
- English.
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In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 214:1, s. 294-303
- Related links:
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https://eprints.whit...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4, 6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax), and five-fold lower Michaelis Constant (Km) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- 2
- 4
- 6-trinitrotoluene
- Arabidopsis
- conjugation
- detoxification
- Drosophila melanogaster
- environmental pollutant
- glutathione transferase
- phytoremediation
Publication and Content Type
- ref (subject category)
- art (subject category)
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