Ultrasound-induced activation of Wnt signaling in human MG-63 osteoblastic cells.

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Ultrasound-induced activation of Wnt signaling in human MG-63 osteoblastic cells.

Olkku, Anu (författare): School of Medicine, Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland

Leskinen, Jarkko (författare): Department of Physics and Mathematics, University of Eastern Finland, Kuopio, Finland

Lammi, Mikko, 1961- (författare): Department of Biosciences, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group

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Hynynen, Kullervo (författare): Department of Physics and Mathematics, University of Eastern Finland, Kuopio, Finland; Department of Medical Biophysics, University of Toronto and Imaging Research, Sunnybrook Health Sciences Centre, Toronto, Canada

Mahonen, Anitta (författare): chool of Medicine, Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital, Finland; Bone and Cartilage Research Unit, University of Eastern Finland, Kuopio, Finland

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Elsevier BV, 2010
2010
Engelska.
Ingår i: Bone. - : Elsevier BV. - 8756-3282 .- 1873-2763. ; 47:2, s. 320-330

Relaterad länk:: http://www.sciencedi...; visa fler...; https://urn.kb.se/re...; https://doi.org/10.1...; visa färre...

Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

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The benefit from an ultrasound (US) exposure for fracture healing has been clearly shown. However, the molecular mechanisms behind this effect are not fully known. Recently, the canonical Wnt signaling pathway has been recognized as one of the essential regulators of osteoblastogenesis and bone mass, and thereby considered crucial for bone health. Mechanical loading and fluid shear stress have been reported to activate the canonical Wnt signaling pathway in bone cells, but previous reports on the effects of therapeutic US on Wnt signaling in general or in bone, in particular, have not been published yet. Therefore, activation of Wnt signaling pathway was assayed in human osteoblastic cells, and indeed, this pathway was found to be activated in MG-63 cells through the phosphoinositol 3-kinase/Akt (PI3K/Akt) and mTOR cascades following a single 10 min US exposure (2 W, 1.035 MHz). In addition to the reporter assay results, the Wnt pathway activation was also observed as nuclear localization of beta-catenin. Wnt activation showed also temperature dependence at elevated temperatures, and the expression of canonical Wnt ligands was induced under the thermal exposures. However, existence of a specific, non-thermal US component was evident as well, perhaps evidence of a potential dual action of therapeutic US on bone. Neither US nor heat exposures affected cell viability in our experiments. In summary, this is the first study to report that Wnt signaling cascade, important for osteoblast function and bone health, is one of the pathways activated by therapeutic US as well as by hyperthermia in human osteoblastic cells. Our results provide evidence for the potential molecular mechanisms behind the beneficial effects of US on fracture healing. Combinations of US, heat, and possible pharmacological treatment could provide useful flexibility for clinical cases in treating various bone disorders.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)

Nyckelord

ultrasound
osteoblast
bone
Wnt signaling
heat
fracture healing
cell research
cellforskning
molekylärbiologi
Molecular Biology
ortopedi
Orthopaedics

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Av författaren/redakt...: Olkku, Anu; Leskinen, Jarkko; Lammi, Mikko, 19 ...; Hynynen, Kullerv ...; Mahonen, Anitta

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Ortopedi

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinsk biotek ...; och Biomedicinsk lab ...

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Av lärosätet: Umeå universitet

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