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Phenotype character...
Phenotype characterisation using integrated gene transcript, protein and metabolite profiling
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- Oresic, Matej, 1967- (författare)
- Beyond Genomics Inc., Waltham, USA; VTT Biotechnology, Espoo, Finland
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- Clish, Clary B. (författare)
- Beyond Genomics Inc., Waltham, USA
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- Davidov, Eugene J. (författare)
- Beyond Genomics Inc., Waltham, USA; Molecular Biophysics and Biochemistry, Yale University, New Haven, USA
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- Verheij, Elwin (författare)
- TNO Pharma, Zeist, The Netherlands
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- Vogels, Jack (författare)
- TNO Pharma, Zeist, The Netherlands
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- Havekes, Louis M. (författare)
- Gaubius Laboratorium, TNO Prevention and Health, Leiden, The Netherlands; Leiden/Amsterdam Center for Drug Research and the Departments of Cardiology and Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands
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- Neumann, Eric (författare)
- Beyond Genomics Inc., Waltham, USA; Aventis, Cambridge, USA
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- Adourian, Aram (författare)
- Beyond Genomics Inc., Waltham, USA
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- Naylor, Stephen (författare)
- Beyond Genomics Inc., Waltham, USA; Boston University School of Medicine, Boston, USA
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- van der Greef, Jan (författare)
- Beyond Genomics Inc., Waltham, USA; TNO Pharma, Zeist, The Netherlands; Leiden/Amsterdam Center for Drug Research and the Departments of Cardiology and Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands
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- Plasterer, Thomas (författare)
- Beyond Genomics Inc., Waltham, USA
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Beyond Genomics Inc, Waltham, USA; VTT Biotechnology, Espoo, Finland Beyond Genomics Inc., Waltham, USA (creator_code:org_t)
- Adis International Ltd. 2004
- 2004
- Engelska.
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Ingår i: Applied bioinformatics. - : Adis International Ltd.. - 1175-5636. ; 3:4, s. 205-217
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
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- Multifactorial diseases present a significant challenge for functional genomics. Owing to their multiple compartmental effects and complex biomolecular activities, such diseases cannot be adequately characterised by changes in single components, nor can pathophysiological changes be understood by observing gene transcripts alone. Instead, a pattern of subtle changes is observed in multifactorial diseases across multiple tissues and organs with complex associations between corresponding gene, protein and metabolite levels. This article presents methods for exploratory and integrative analysis of pathophysiological changes at the biomolecular level. In particular, novel approaches are introduced for the following challenges: (i) data processing and analysis methods for proteomic and metabolomic data obtained by electrospray ionisation (ESI) liquid chromatography-tandem mass spectrometry (LC/MS); (ii) association analysis of integrated gene, protein and metabolite patterns that are most descriptive of pathophysiological changes; and (iii) interpretation of results obtained from association analyses in the context of known biological processes. These novel approaches are illustrated with the apolipoprotein E3-Leiden transgenic mouse model, a commonly used model of atherosclerosis. We seek to gain insight into the early responses of disease onset and progression by determining and identifying--well in advance of pathogenic manifestations of disease--the sets of gene transcripts, proteins and metabolites, along with their putative relationships in the transgenic model and associated wild-type cohort. Our results corroborate previous findings and extend predictions for three processes in atherosclerosis: aberrant lipid metabolism, inflammation, and tissue development and maintenance.
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- Enzyme Commission
- Kernel Principal Component Analysis
- Normal Chow Diet
- Bioanalytical Platform
- Biomolecular Component
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Oresic, Matej, 1 ...
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Clish, Clary B.
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Davidov, Eugene ...
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Verheij, Elwin
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Vogels, Jack
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Havekes, Louis M ...
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visa fler...
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Neumann, Eric
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Adourian, Aram
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Naylor, Stephen
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van der Greef, J ...
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Plasterer, Thoma ...
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- NATURVETENSKAP
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Applied bioinfor ...
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Örebro universitet