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Gamma-tubulin coord...
Gamma-tubulin coordinates nuclear envelope assembly around chromatin
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- Rossello Castillo, Catalina Ana (författare)
- Lund University,Lunds universitet,Molekylär patologi, Malmö,Forskargrupper vid Lunds universitet,Molecular Pathology, Malmö,Lund University Research Groups,Skåne University Hospital
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- Lindström, Lisa (författare)
- Lund University,Lunds universitet,Molekylär patologi, Malmö,Forskargrupper vid Lunds universitet,Molecular Pathology, Malmö,Lund University Research Groups,Skåne University Hospital
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- Glindre, Johan (författare)
- Lund University,Lunds universitet,Molekylär patologi, Malmö,Forskargrupper vid Lunds universitet,Molecular Pathology, Malmö,Lund University Research Groups,Skåne University Hospital
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- Eklund, Greta (författare)
- Lund University,Lunds universitet,Molekylär patologi, Malmö,Forskargrupper vid Lunds universitet,Molecular Pathology, Malmö,Lund University Research Groups,Skåne University Hospital
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- Alvarado-Kristensson, Maria (författare)
- Lund University,Lunds universitet,Molekylär patologi, Malmö,Forskargrupper vid Lunds universitet,Molecular Pathology, Malmö,Lund University Research Groups,Skåne University Hospital
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(creator_code:org_t)
- Elsevier BV, 2016
- 2016
- Engelska.
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Ingår i: Heliyon. - : Elsevier BV. - 2405-8440. ; 2:9
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Biological sciences
- Cell biology
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