WFRF:(Shilkova Olga)
Sökning: WFRF:(Shilkova Olga) > P-TEFb, the Super E...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03682naa a2200373 4500 | |
| 001 | oai:DiVA.org:su-117046 | |
| 003 | SwePub | |
| 008 | 150506s2015 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1170462 URI |
| 024 | 7 | a https://doi.org/10.1371/journal.pgen.10049712 DOI |
| 040 | a (SwePub)su | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Dahlberg, Olleu Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut0 (Swepub:su)odahl |
| 245 | 1 0 | a P-TEFb, the Super Elongation Complex and Mediator Regulate a Subset of Non-paused Genes during Early Drosophila Embryo Development |
| 264 | c 2015-02-13 | |
| 264 | 1 | b Public Library of Science (PLoS),c 2015 |
| 338 | a print2 rdacarrier | |
| 500 | a AuthorCount:5; | |
| 520 | a Positive Transcription Elongation Factor b (P-TEFb) is a kinase consisting of Cdk9 and Cyclin T that releases RNA Polymerase II (Pol II) into active elongation. It can assemble into a larger Super Elongation Complex (SEC) consisting of additional elongation factors. Here, we use a miRNA-based approach to knock down the maternal contribution of P-TEFb and SEC components in early Drosophila embryos. P-TEFb or SEC depletion results in loss of cells from the embryo posterior and in cellularization defects. Interestingly, the expression of many patterning genes containing promoter-proximal paused Pol II is relatively normal in P-TEFb embryos. Instead, P-TEFb and SEC are required for expression of some non-paused, rapidly transcribed genes in pre-cellular embryos, including the cellularization gene Serendipity-a. We also demonstrate that another P-TEFb regulated gene, terminus, has an essential function in embryo development. Similar morphological and gene expression phenotypes were observed upon knock down of Mediator subunits, providing in vivo evidence that P-TEFb, the SEC and Mediator collaborate in transcription control. Surprisingly, P-TEFb depletion does not affect the ratio of Pol II at the promoter versus the 3' end, despite affecting global Pol II Ser2 phosphorylation levels. Instead, Pol II occupancy is reduced at P-TEFb down-regulated genes. We conclude that a subset of non-paused, pre-cellular genes are among the most susceptible to reduced P-TEFb, SEC and Mediator levels in Drosophila embryos. | |
| 650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
| 700 | 1 | a Shilkova, Olgau Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut |
| 700 | 1 | a Tang, Minu Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,South China University, China4 aut0 (Swepub:su)mtang |
| 700 | 1 | a Holmqvist, Per-Henriku Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut0 (Swepub:su)perho |
| 700 | 1 | a Mannervik, Mattiasu Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut0 (Swepub:su)maman |
| 710 | 2 | a Stockholms universitetb Institutionen för molekylär biovetenskap, Wenner-Grens institut4 org |
| 773 | 0 | t PLOS Geneticsd : Public Library of Science (PLoS)g 11:2q 11:2x 1553-7390x 1553-7404 |
| 856 | 4 | u https://doi.org/10.1371/journal.pgen.1004971y Fulltext |
| 856 | 4 | u https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004971&type=printable |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-117046 |
| 856 | 4 8 | u https://doi.org/10.1371/journal.pgen.1004971 |
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