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Ablation of the Cha...
Ablation of the Chaperone Protein ERdj5 Results in a Sjogrens Syndrome-Like Phenotype in Mice, Consistent With an Upregulated Unfolded Protein Response in Human Patients
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- Apostolou, Eirini (author)
- Univ Athens, Greece; Univ Athens, Greece
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- Moustardas, Petros (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för klinisk kemi,Acad Athens, Greece
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- Iwawaki, Takao (author)
- Kanazawa Med Univ, Japan
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- Tzioufas, Athanasios G. (author)
- Univ Athens, Greece; Univ Athens, Greece
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- Spyrou, Ioannis (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för klinisk kemi
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(creator_code:org_t)
- 2019-03-22
- 2019
- English.
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In: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 10
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https://www.frontier...
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https://doi.org/10.3...
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Abstract
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- Objective: Sjogrens syndrome (SS) is a chronic autoimmune disorder that affects mainly the exocrine glands. Endoplasmic reticulum (ER) stress proteins have been suggested to participate in autoimmune and inflammatory responses, either acting as autoantigens, or by modulating factors of inflammation. The chaperone protein ERdj5 is an ER-resident disulfide reductase, required for the translocation of misfolded proteins during ER-associated protein degradation. In this study we investigated the role of ERdj5 in the salivary glands (SGs), in association with inflammation and autoimmunity. Methods: In situ expression of ERdj5 and XBP1 activation were studied immunohistochemically in minor SG tissues from primary SS patients and non-SS sicca-complaining controls. We used the mouse model of ERdj5 ablation and characterized its features: Histopathological, serological (antinuclear antibodies and cytokine levels), and functional (saliva flow rate). Results: ERdj5 was highly expressed in the minor SGs of SS patients, with stain intensity correlated to inflammatory lesion severity and anti-SSA/Ro positivity. Moreover, SS patients demonstrated higher XBP1 activation within the SGs. Remarkably, ablation of ERdj5 in mice conveyed many of the cardinal features of SS, like spontaneous inflammation in SGs with infiltrating T and B lymphocytes, distinct cytokine signature, excessive cell death, reduced saliva flow, and production of anti-SSA/Ro and anti-SSB/La autoantibodies. Notably, these features were more pronounced in female mice. Conclusions: Our findings suggest a critical connection between the function of the ER chaperone protein ERdj5 and autoimmune inflammatory responses in the SGs and provide evidence for a new, potent animal model of SS.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- ERdj5; ER-stress; autoimmunity; salivary gland; Sjogrens syndrome; XBP1; UPR
Publication and Content Type
- ref (subject category)
- art (subject category)
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