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WFRF:(Takada Hiraku)
 

Sökning: WFRF:(Takada Hiraku) > (2022) > Expression of Bacil...

Expression of Bacillus subtilis ABCF antibiotic resistance factor VmlR is regulated by RNA polymerase pausing, transcription attenuation, translation attenuation and (p)ppGpp

Takada, Hiraku (författare)
Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Faculty of Life Sciences, Kyoto Sangyo University, Institute for Protein Dynamics, Kamigamo, Motoyama, Kita-ku, Kyoto, Japan; Department of Experimental Medical Science, Lund University, Lund, Sweden,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
Mandell, Zachary F. (författare)
Department of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, PA, University Park, United States
Yakhnin, Helen (författare)
Department of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, PA, University Park, United States
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Glazyrina, Anastasiya (författare)
Umeå University,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
Chiba, Shinobu (författare)
Faculty of Life Sciences, Kyoto Sangyo University, Institute for Protein Dynamics, Kamigamo, Motoyama, Kita-ku, Kyoto, Japan
Kurata, Tatsuaki (författare)
Lund University,Lunds universitet,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
Wu, Kelvin J.Y. (författare)
Department of Chemistry and Chemical Biology, Harvard University, MA, Cambridge, United States
Tresco, Ben I.C. (författare)
Department of Chemistry and Chemical Biology, Harvard University, MA, Cambridge, United States
Myers, Andrew G. (författare)
Department of Chemistry and Chemical Biology, Harvard University, MA, Cambridge, United States
Atkinson, Gemma (författare)
Lund University,Lunds universitet,Proteinevolution,Forskargrupper vid Lunds universitet,Protein Evolution,Lund University Research Groups
Babitzke, Paul (författare)
Department of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, PA, University Park, United States
Hauryliuk, Vasili, 1980- (författare)
Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medical Science, Lund University, Lund, Sweden; University of Tartu, Institute of Technology, Tartu, Estonia,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
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 (creator_code:org_t)
2022-06-14
2022
Engelska.
Ingår i: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 50:11, s. 6174-6189
Relaterad länk:
https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
http://dx.doi.org/10... (free)
https://urn.kb.se/re...
https://doi.org/10.1...
https://lup.lub.lu.s...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Since antibiotic resistance is often associated with a fitness cost, bacteria employ multi-layered regulatory mechanisms to ensure that expression of resistance factors is restricted to times of antibiotic challenge. In Bacillus subtilis, the chromosomally-encoded ABCF ATPase VmlR confers resistance to pleuromutilin, lincosamide and type A streptogramin translation inhibitors. Here we show that vmlR expression is regulated by translation attenuation and transcription attenuation mechanisms. Antibiotic-induced ribosome stalling during translation of an upstream open reading frame in the vmlR leader region prevents formation of an anti-antiterminator structure, leading to the formation of an antiterminator structure that prevents intrinsic termination. Thus, transcription in the presence of antibiotic induces vmlR expression. We also show that NusG-dependent RNA polymerase pausing in the vmlR leader prevents leaky expression in the absence of antibiotic. Furthermore, we demonstrate that induction of VmlR expression by compromised protein synthesis does not require the ability of VmlR to rescue the translational defect, as exemplified by constitutive induction of VmlR by ribosome assembly defects. Rather, the specificity of induction is determined by the antibiotic's ability to stall the ribosome on the regulatory open reading frame located within the vmlR leader. Finally, we demonstrate the involvement of (p)ppGpp-mediated signalling in antibiotic-induced VmlR expression.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

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