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LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 05950naa a2201537 4500 | |
| 001 | oai:DiVA.org:miun-41548 | |
| 003 | SwePub | |
| 008 | 210310s2020 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-415482 URI |
| 024 | 7 | a https://doi.org/10.1038/s41467-020-18156-72 DOI |
| 040 | a (SwePub)miun | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Echelmeier, A.4 aut |
| 245 | 1 0 | a Segmented flow generator for serial crystallography at the European X-ray free electron laser |
| 264 | c 2020-09-09 | |
| 264 | 1 | b Nature Research,c 2020 |
| 338 | a print2 rdacarrier | |
| 520 | a Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported. | |
| 650 | 7 | a NATURVETENSKAPx Fysikx Atom- och molekylfysik och optik0 (SwePub)103022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Physical Sciencesx Atom and Molecular Physics and Optics0 (SwePub)103022 hsv//eng |
| 653 | a crystallography | |
| 653 | a droplet | |
| 653 | a enzyme activity | |
| 653 | a high pressure | |
| 653 | a laser method | |
| 653 | a segmentation | |
| 653 | a water treatment | |
| 653 | a aqueous solution | |
| 653 | a Article | |
| 653 | a conformational transition | |
| 653 | a controlled study | |
| 653 | a crystal structure | |
| 653 | a high performance liquid chromatography | |
| 653 | a physical parameters | |
| 653 | a pulse rate | |
| 653 | a synchrotron radiation | |
| 653 | a three dimensional printing | |
| 653 | a X ray crystallography | |
| 653 | a devices | |
| 653 | a electron | |
| 653 | a hydrodynamics | |
| 653 | a lab on a chip | |
| 653 | a laser | |
| 653 | a ultrastructure | |
| 653 | a 2-dehydro-3-deoxyphosphooctonate aldolase | |
| 653 | a Escherichia coli protein | |
| 653 | a lyase | |
| 653 | a Aldehyde-Lyases | |
| 653 | a Electrons | |
| 653 | a Escherichia coli Proteins | |
| 653 | a Lab-On-A-Chip Devices | |
| 653 | a Lasers | |
| 700 | 1 | a Cruz Villarreal, J.4 aut |
| 700 | 1 | a Messerschmidt, M.4 aut |
| 700 | 1 | a Kim, D.4 aut |
| 700 | 1 | a Coe, J. D.4 aut |
| 700 | 1 | a Thifault, D.4 aut |
| 700 | 1 | a Botha, S.4 aut |
| 700 | 1 | a Egatz-Gomez, A.4 aut |
| 700 | 1 | a Gandhi, S.4 aut |
| 700 | 1 | a Brehm, G.4 aut |
| 700 | 1 | a Conrad, C. E.4 aut |
| 700 | 1 | a Hansen, D. T.4 aut |
| 700 | 1 | a Madsen, C.4 aut |
| 700 | 1 | a Bajt, S.4 aut |
| 700 | 1 | a Meza-Aguilar, J. D.4 aut |
| 700 | 1 | a Oberthür, D.4 aut |
| 700 | 1 | a Wiedorn, M. O.4 aut |
| 700 | 1 | a Fleckenstein, H.4 aut |
| 700 | 1 | a Mendez, D.4 aut |
| 700 | 1 | a Knoška, J.4 aut |
| 700 | 1 | a Martin-Garcia, J. M.4 aut |
| 700 | 1 | a Hu, H.4 aut |
| 700 | 1 | a Lisova, S.4 aut |
| 700 | 1 | a Allahgholi, A.4 aut |
| 700 | 1 | a Gevorkov, Y.4 aut |
| 700 | 1 | a Ayyer, K.4 aut |
| 700 | 1 | a Aplin, S.4 aut |
| 700 | 1 | a Ginn, H. M.4 aut |
| 700 | 1 | a Graafsma, H.u Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany4 aut0 (Swepub:miun)exheigra |
| 700 | 1 | a Morgan, A. J.4 aut |
| 700 | 1 | a Greiffenberg, D.4 aut |
| 700 | 1 | a Klujev, A.4 aut |
| 700 | 1 | a Laurus, T.4 aut |
| 700 | 1 | a Poehlsen, J.4 aut |
| 700 | 1 | a Trunk, U.4 aut |
| 700 | 1 | a Mezza, D.4 aut |
| 700 | 1 | a Schmidt, B.4 aut |
| 700 | 1 | a Kuhn, M.4 aut |
| 700 | 1 | a Fromme, R.4 aut |
| 700 | 1 | a Sztuk-Dambietz, J.4 aut |
| 700 | 1 | a Raab, N.4 aut |
| 700 | 1 | a Hauf, S.4 aut |
| 700 | 1 | a Silenzi, A.4 aut |
| 700 | 1 | a Michelat, T.4 aut |
| 700 | 1 | a Xu, C.4 aut |
| 700 | 1 | a Danilevski, C.4 aut |
| 700 | 1 | a Parenti, A.4 aut |
| 700 | 1 | a Mekinda, L.4 aut |
| 700 | 1 | a Weinhausen, B.4 aut |
| 700 | 1 | a Mills, G.4 aut |
| 700 | 1 | a Vagovic, P.4 aut |
| 700 | 1 | a Kim, Y.4 aut |
| 700 | 1 | a Kirkwood, H.4 aut |
| 700 | 1 | a Bean, R.4 aut |
| 700 | 1 | a Bielecki, J.4 aut |
| 700 | 1 | a Stern, S.4 aut |
| 700 | 1 | a Giewekemeyer, K.4 aut |
| 700 | 1 | a Round, A. R.4 aut |
| 700 | 1 | a Schulz, J.4 aut |
| 700 | 1 | a Dörner, K.4 aut |
| 700 | 1 | a Grant, T. D.4 aut |
| 700 | 1 | a Mariani, V.4 aut |
| 700 | 1 | a Barty, A.4 aut |
| 700 | 1 | a Mancuso, A. P.4 aut |
| 700 | 1 | a Weierstall, U.4 aut |
| 700 | 1 | a Spence, J. C. H.4 aut |
| 700 | 1 | a Chapman, H. N.4 aut |
| 700 | 1 | a Zatsepin, N.4 aut |
| 700 | 1 | a Fromme, P.4 aut |
| 700 | 1 | a Kirian, R. A.4 aut |
| 700 | 1 | a Ros, A.4 aut |
| 710 | 2 | a Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany4 org |
| 773 | 0 | t Nature Communicationsd : Nature Researchg 11:1q 11:1x 2041-1723 |
| 856 | 4 | u https://doi.org/10.1038/s41467-020-18156-7y Fulltext |
| 856 | 4 | u https://www.nature.com/articles/s41467-020-18156-7.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-41548 |
| 856 | 4 8 | u https://doi.org/10.1038/s41467-020-18156-7 |
Hitta mer i SwePub
- Av författaren/redakt...
- Echelmeier, A.
- Cruz Villarreal, ...
- Messerschmidt, M ...
- Kim, D.
- Coe, J. D.
- Thifault, D.
- visa fler...
- Botha, S.
- Egatz-Gomez, A.
- Gandhi, S.
- Brehm, G.
- Conrad, C. E.
- Hansen, D. T.
- Madsen, C.
- Bajt, S.
- Meza-Aguilar, J. ...
- Oberthür, D.
- Wiedorn, M. O.
- Fleckenstein, H.
- Mendez, D.
- Knoška, J.
- Martin-Garcia, J ...
- Hu, H.
- Lisova, S.
- Allahgholi, A.
- Gevorkov, Y.
- Ayyer, K.
- Aplin, S.
- Ginn, H. M.
- Graafsma, H.
- Morgan, A. J.
- Greiffenberg, D.
- Klujev, A.
- Laurus, T.
- Poehlsen, J.
- Trunk, U.
- Mezza, D.
- Schmidt, B.
- Kuhn, M.
- Fromme, R.
- Sztuk-Dambietz, ...
- Raab, N.
- Hauf, S.
- Silenzi, A.
- Michelat, T.
- Xu, C.
- Danilevski, C.
- Parenti, A.
- Mekinda, L.
- Weinhausen, B.
- Mills, G.
- Vagovic, P.
- Kim, Y.
- Kirkwood, H.
- Bean, R.
- Bielecki, J.
- Stern, S.
- Giewekemeyer, K.
- Round, A. R.
- Schulz, J.
- Dörner, K.
- Grant, T. D.
- Mariani, V.
- Barty, A.
- Mancuso, A. P.
- Weierstall, U.
- Spence, J. C. H.
- Chapman, H. N.
- Zatsepin, N.
- Fromme, P.
- Kirian, R. A.
- Ros, A.
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