SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Wennborg Anders)
 

Sökning: WFRF:(Wennborg Anders) > (2015-2019) > Intra-image referen...

Intra-image referencing for simplified assessment of HER2-expression in breast cancer metastases using the Affibody molecule ABY-025 with PET and SPECT.

Sandberg, Dan T (författare)
Uppsala universitet,Medicinsk strålningsvetenskap
Tolmachev, Vladimir (författare)
Uppsala universitet,Medicinsk strålningsvetenskap
Velikyan, Irina, 1966- (författare)
Uppsala universitet,Radiologi
visa fler...
Olofsson, Helena (författare)
Uppsala universitet,Klinisk och experimentell patologi
Wennborg, Anders (författare)
Affibody AB, Solna, Sweden
Feldwisch, Joachim (författare)
Affibody AB, Solna, Sweden
Carlsson, Jörgen, 1944- (författare)
Uppsala universitet,Medicinsk strålningsvetenskap
Lindman, Henrik (författare)
Uppsala universitet,Experimentell och klinisk onkologi
Sörensen, Jens (författare)
Uppsala universitet,Radiologi
visa färre...
 (creator_code:org_t)
2017-03-06
2017
Engelska.
Ingår i: European Journal of Nuclear Medicine and Molecular Imaging. - : Springer Science and Business Media LLC. - 1619-7070 .- 1619-7089. ; 44:8, s. 1337-1346
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • PURPOSE: In phase I/II-studies radiolabelled ABY-025 Affibody molecules identified human epidermal growth factor receptor 2 (HER2) expression in breast cancer metastases using PET and SPECT imaging. Here, we wanted to investigate the utility of a simple intra-image normalization using tumour-to-reference tissue-ratio (T/R) as a HER2 status discrimination strategy to overcome potential issues related to cross-calibration of scanning devices.METHODS: Twenty-three women with pre-diagnosed HER2-positive/negative metastasized breast cancer were scanned with [(111)In]-ABY-025 SPECT/CT (n = 7) or [(68)Ga]-ABY-025 PET/CT (n = 16). Uptake was measured in all metastases and in normal spleen, lung, liver, muscle, and blood pool. Normal tissue uptake variation and T/R-ratios were established for various time points and for two different doses of injected peptide from a total of 94 whole-body image acquisitions. Immunohistochemistry (IHC) was used to verify HER2 expression in 28 biopsied metastases. T/R-ratios were compared to IHC findings to establish the best reference tissue for each modality and each imaging time-point. The impact of shed HER2 in serum was investigated.RESULTS: Spleen was the best reference tissue across modalities, followed by blood pool and lung. Spleen-T/R was highly correlated to PET SUV in metastases after 2 h (r = 0.96, P < 0.001) and reached an accuracy of 100% for discriminating IHC HER2-positive and negative metastases at 4 h (PET) and 24 h (SPECT) after injection. In a single case, shed HER2 resulted in intense tracer retention in blood. In the remaining patients shed HER2 was elevated, but without significant impact on ABY-025 biodistribution.CONCLUSION: T/R-ratios using spleen as reference tissue accurately quantify HER2 expression with radiolabelled ABY-025 imaging in breast cancer metastases with SPECT and PET. Tracer binding to shed HER2 in serum might affect quantification in the extreme case.

Nyckelord

Affibody
HER2-receptor
PET
SPECT
Shedding
T/R

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy