Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential

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Fältnamn	Indikatorer	Metadata
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001		oai:DiVA.org:umu-183242
003		SwePub
008		210519s2021 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:uu-458519
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1832422 URI
024	7	a https://doi.org/10.1016/j.bjps.2021.03.0252 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4585192 URI
040		a (SwePub)umud (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Lauvrud, Anne Thereseu Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Handkirurgi,Umeå Univ, Dept Integrat Med Biol, Umeå, Sweden.; Umeå Univ, Dept Surg & Perioperat Sci, S-90737 Umeå, Sweden4 aut0 (Swepub:umu)anth0067
245	1 0	a Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential
264	1	b Elsevier,c 2021
338		a electronic2 rdacarrier
520		a Introduction: Water jet-assisted liposuction has gained popularity due to favourable fat grafting outcomes. In this study, we compared stem cells obtained from fat isolated with manual or the water jet-assisted procedure.Methods: Liposuction of abdominal fat was performed using the two methods on each donor (n = 10). Aspirate samples were collagenase digested and the isolated cells seeded in vitro prior to proliferation, adipogenic differentiation and angiogenic activity analyses.Results: Cells from either procedure proliferated at similar rates and exhibited a similar colony-forming ability. The cells expressed stem cell markers CD73, CD90 and CD105. In the water jet cell preparations, there were higher numbers of cells expressing CD146. Robust adipogenic differentiation was observed in cultures expanded from both manual and water jet lipoaspirates. Gene analysis showed higher expression of the adipocyte markers aP2 and GLUT4 in the adipocyte-differentiated water jet cell preparations, and ELISA indicated increased secretion of adiponectin from these cells. Both cell groups expressed vasculogenic factors and the water jet cells promoted the highest levels of in vitro angiogenesis. Given these positive results, we further characterised the water jet cells when prepared using an automated closed cell processing unit, the Sepax-2 system (Cytiva). The growth and stem cell properties of the Sepax-processed cells were similar to the standard centrifugation protocol, but there was evidence for greater adipogenic differentiation in the Sepax-processed cells.Conclusions: Water jet lipoaspirates yield cells with high adipogenic potential and angiogenic activity, which may be beneficial for use in cell-assisted lipotransfers.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Kirurgi0 (SwePub)302122 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Surgery0 (SwePub)302122 hsv//eng
653		a adipogenesis
653		a angiogenesis
653		a differentiation
653		a fat grafting
653		a stem cells
700	1	a Gümüsçü, Rojdau Uppsala universitet,Umeå universitet,Handkirurgi,Plastikkirurgi,Umeå Univ, Dept Surg & Perioperat Sci, S-90737 Umeå, Sweden4 aut0 (Swepub:uu)rojgu356
700	1	a Wiberg, Rebecca,d 1988-u Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Handkirurgi,Umeå Univ, Dept Integrat Med Biol, Umeå, Sweden.; Umeå Univ, Dept Surg & Perioperat Sci, S-90737 Umeå, Sweden4 aut0 (Swepub:umu)revi0003
700	1	a Brohlin, Maria,d 1966-u Umeå universitet,Institutionen för klinisk mikrobiologi,Umeå Univ, Dept Clin Microbiol Infect & Immun, Umeå, Sweden4 aut0 (Swepub:umu)mabr0001
700	1	a Kelk, Peymanu Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå Univ, Dept Integrat Med Biol, Umeå, Sweden4 aut0 (Swepub:umu)kepe0001
700	1	a Wiberg, Mikaelu Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Handkirurgi,Umeå Univ, Dept Integrat Med Biol, Umeå, Sweden.; Umeå Univ, Dept Surg & Perioperat Sci, S-90737 Umeå, Sweden4 aut0 (Swepub:umu)miwi0004
700	1	a Kingham, Paul J.u Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå Univ, Dept Integrat Med Biol, Umeå, Sweden4 aut0 (Swepub:umu)paki0006
710	2	a Umeå universitetb Institutionen för integrativ medicinsk biologi (IMB)4 org
773	0	t Journal of Plastic, Reconstructive & Aesthetic Surgeryd : Elsevierg 74:10, s. 2759-2767q 74:10<2759-2767x 1748-6815x 1878-0539
856	4	u https://doi.org/10.1016/j.bjps.2021.03.025y Fulltext
856	4	u https://umu.diva-portal.org/smash/get/diva2:1555960/FULLTEXT02.pdfx primaryx Raw objecty fulltext:print
856	4	u http://www.jprasurg.com/article/S1748681521001157/pdf
856	4	u https://uu.diva-portal.org/smash/get/diva2:1610546/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183242
856	4 8	u https://doi.org/10.1016/j.bjps.2021.03.025
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-458519

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Av författaren/redakt...: Lauvrud, Anne Th ...; Gümüsçü, Rojda; Wiberg, Rebecca, ...; Brohlin, Maria, ...; Kelk, Peyman; Wiberg, Mikael; visa fler...; Kingham, Paul J.; visa färre...

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