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WFRF:(Zhang Dongfeng)
 

Sökning: WFRF:(Zhang Dongfeng) > Glioma Cell Prolife...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00007201naa a2200529 4500
001oai:lup.lub.lu.se:feaab906-36ca-40a8-b717-e576e8940d47
003SwePub
008160401s2014 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/43358222 URI
0247 a https://doi.org/10.1371/journal.pone.00872812 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Chen, Dongfengu Lund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rausinglaboratoriet i Lund - Tumörsektionen,Forskargrupper vid Lunds universitet,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Rausing laboratory of Lund - Tumor section,Lund University Research Groups4 aut0 (Swepub:lu)med-dcn
2451 0a Glioma Cell Proliferation Controlled by ERK Activity-Dependent Surface Expression of PDGFRA.
264 c 2014-01-29
264 1b Public Library of Science (PLoS),c 2014
338 a electronic2 rdacarrier
520 a Increased PDGFRA signaling is an essential pathogenic factor in many subtypes of gliomas. In this context the cell surface expression of PDGFRA is an important determinant of ligand sensing in the glioma microenvironment. However, the regulation of spatial distribution of PDGFRA in glioma cells remains poorly characterized. Here, we report that cell surface PDGFRA expression in gliomas is negatively regulated by an ERK-dependent mechanism, resulting in reduced proliferation of glioma cells. Glioma tumor tissues and their corresponding cell lines were isolated from 14 patients and analyzed by single-cell imaging and flow cytometry. In both cell lines and their corresponding tumor samples, glioma cell proliferation correlated with the extent of surface expression of PDGFRA. High levels of surface PDGFRA also correlated to high tubulin expression in glioma tumor tissue in vivo. In glioma cell lines, surface PDGFRA declined following treatment with inhibitors of tubulin, actin and dynamin. Screening of a panel of small molecule compounds identified the MEK inhibitor U0126 as a potent inhibitor of surface PDGFRA expression. Importantly, U0126 inhibited surface expression in a reversible, dose- and time-dependent manner, without affecting general PDGFRA expression. Treatment with U0126 resulted in reduced co-localization between PDGFRA and intracellular trafficking molecules e.g. clathrin, RAB11 and early endosomal antigen-1, in parallel with enhanced co-localization between PDGFRA and the Golgi cisternae maker, Giantin, suggesting a deviation of PDGFRA from the endosomal trafficking and recycling compartment, to the Golgi network. Furthermore, U0126 treatment in glioma cells induced an initial inhibition of ERK1/2 phosphorylation, followed by up-regulated ERK1/2 phosphorylation concomitant with diminished surface expression of PDGFRA. Finally, down-regulation of surface PDGFRA expression by U0126 is concordant with reduced glioma cell proliferation. These findings suggest that manipulation of spatial expression of PDGFRA can potentially be used to combat gliomas.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Neurologi0 (SwePub)302072 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Neurology0 (SwePub)302072 hsv//eng
7001 a Zuo, Duou Beijing Normal University4 aut
7001 a Luan, Chengu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups4 aut0 (Swepub:lu)med-cgl
7001 a Min, Liuu Lund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rausinglaboratoriet i Lund - Tumörsektionen,Forskargrupper vid Lunds universitet,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Rausing laboratory of Lund - Tumor section,Lund University Research Groups4 aut0 (Swepub:lu)med-lum
7001 a Na, Manliu Lund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rausinglaboratoriet i Lund - Tumörsektionen,Forskargrupper vid Lunds universitet,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Rausing laboratory of Lund - Tumor section,Lund University Research Groups4 aut0 (Swepub:lu)med-min
7001 a Ran, Liangu Beijing Normal University4 aut
7001 a Sun, Yingyuu Beijing Normal University4 aut
7001 a Persson, Annetteu Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)pat-ape
7001 a Englund, Elisabetu Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)pat-een
7001 a Salford, Leifu Lund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rausinglaboratoriet i Lund - Tumörsektionen,Forskargrupper vid Lunds universitet,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Rausing laboratory of Lund - Tumor section,Lund University Research Groups4 aut0 (Swepub:lu)nkir-lsa
7001 a Renström, Eriku Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-ere
7001 a Fan, Xiaolongu Beijing Normal University4 aut0 (Swepub:lu)molm-xfa
7001 a Zhang, Enmingu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups4 aut0 (Swepub:lu)med-ezg
7102 a Neurokirurgib Sektion IV4 org
7730 t PLoS ONEd : Public Library of Science (PLoS)g 9:1q 9:1x 1932-6203
8564 u https://portal.research.lu.se/files/4058450/4779729.pdfx primaryx freey FULLTEXT
8564 u http://www.ncbi.nlm.nih.gov/pubmed/24489888?dopt=Abstracty FULLTEXT
8564 u http://dx.doi.org/10.1371/journal.pone.0087281x freey FULLTEXT
8564 u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0087281&type=printable
8564 8u https://lup.lub.lu.se/record/4335822
8564 8u https://doi.org/10.1371/journal.pone.0087281

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