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Spider Silk Protein...
Spider Silk Protein Forms Amyloid-Like Nanofibrils through a Non-Nucleation-Dependent Polymerization Mechanism (Small 46/2023)
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- Qi, Xingmei (författare)
- Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China.,The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China
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- Wang, Yu (författare)
- Karolinska Inst, Dept Biosci & Nutr, S-14157 Huddinge, Sweden.;Northeast Forestry Univ, Coll Wildlife & Protected Area, Harbin 150040, Peoples R China.,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
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- Yu, Hairui (författare)
- Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China.,The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China
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visa fler...
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- Liu, Ruifang (författare)
- Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China.,The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China
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- Leppert, Axel (författare)
- Karolinska Institutet
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- Zheng, Zihan (författare)
- Karolinska Inst, Dept Biosci & Nutr, S-14157 Huddinge, Sweden.;Xi An Jiao Tong Univ, Dept Pharmacol, Xian 710061, Shaanxi, Peoples R China.,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; Department of Pharmacology, Xi'an Jiaotong University, Shaanxi, China
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- Zhong, Xueying (författare)
- KTH,Strukturell bioteknik,School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Huddinge, Sweden
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- Jin, Zhen (författare)
- Karolinska Inst, Dept Biosci & Nutr, S-14157 Huddinge, Sweden.;Xi An Jiao Tong Univ, Dept Pharmacol, Xian 710061, Shaanxi, Peoples R China.,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; Department of Pharmacology, Xi'an Jiaotong University, Shaanxi, China
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- Wang, Han (författare)
- Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China.,The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China
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- Li, Xiaoli (författare)
- Chongqing Med Univ, Dept Pharmacol, Coll Pharm, Chongqing 400016, Peoples R China.,Department of Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing, China
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- Wang, Xiuzhe (författare)
- Shanghai Jiao Tong Univ, Dept Neurol, Sch Med, Shanghai Peoples Hosp 6, Shanghai 200233, Peoples R China.,Department of Neurology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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- Landreh, Michael (författare)
- Karolinska Institutet
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- Morozova-Roche, Ludmilla A. (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå Univ, Dept Med Biochem & Biophys, S-90187 Umeå, Sweden.
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- Johansson, Jan (författare)
- Karolinska Institutet
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- Xiong, Sidong (författare)
- Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China.,The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China
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- Iashchishyn, Igor (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå Univ, Dept Med Biochem & Biophys, S-90187 Umeå, Sweden.
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- Chen, Gefei (författare)
- Karolinska Institutet
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Soochow Univ, Jiangsu Key Lab Infect & Immun, Inst Biol & Med Sci, Suzhou 215123, Peoples R China The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China (creator_code:org_t)
- Wiley, 2023
- 2023
- Engelska.
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 19:46
- Relaterad länk:
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https://doi.org/10.1...
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visa fler...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Amyloid fibrils—nanoscale fibrillar aggregates with high levels of order—are pathogenic in some today incurable human diseases; however, there are also many physiologically functioning amyloids in nature. The process of amyloid formation is typically nucleation-elongation-dependent, as exemplified by the pathogenic amyloid-β peptide (Aβ) that is associated with Alzheimer's disease. Spider silk, one of the toughest biomaterials, shares characteristics with amyloid. In this study, it is shown that forming amyloid-like nanofibrils is an inherent property preserved by various spider silk proteins (spidroins). Both spidroins and Aβ capped by spidroin N- and C-terminal domains, can assemble into macroscopic spider silk-like fibers that consist of straight nanofibrils parallel to the fiber axis as observed in native spider silk. While Aβ forms amyloid nanofibrils through a nucleation-dependent pathway and exhibits strong cytotoxicity and seeding effects, spidroins spontaneously and rapidly form amyloid-like nanofibrils via a non-nucleation-dependent polymerization pathway that involves lateral packing of fibrils. Spidroin nanofibrils share amyloid-like properties but lack strong cytotoxicity and the ability to self-seed or cross-seed human amyloidogenic peptides. These results suggest that spidroins´ unique primary structures have evolved to allow functional properties of amyloid, and at the same time direct their fibrillization pathways to avoid formation of cytotoxic intermediates.
Ämnesord
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- cytotoxicity
- nanofibril
- non-nucleation-dependent polymerization
- seeding
- spidroin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Qi, Xingmei
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Wang, Yu
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Yu, Hairui
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Liu, Ruifang
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Leppert, Axel
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Zheng, Zihan
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visa fler...
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Zhong, Xueying
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Jin, Zhen
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Wang, Han
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Li, Xiaoli
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Wang, Xiuzhe
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Landreh, Michael
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Morozova-Roche, ...
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Johansson, Jan
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Xiong, Sidong
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Iashchishyn, Igo ...
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Chen, Gefei
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- NATURVETENSKAP
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och Biologi
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NATURVETENSKAP
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Kungliga Tekniska Högskolan
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Umeå universitet
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