Defining the human gallbladder proteome by transcriptomics and affinity proteomics

Kampf, Caroline (författare): Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Uppsala University

Mardinoglu, Adil, 1982 (författare): Chalmers tekniska högskola,Chalmers University of Technology

Fagerberg, Linn (författare): KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)

Hallström, Björn M. (författare): KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)

Danielsson, Angelika (författare): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Uppsala University

Nielsen, Jens (författare): KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Chalmers University of Technology, Sweden,Chalmers tekniska högskola

Pontén, Fredrik (författare): Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Uppsala University

Uhlén, Mathias (författare): KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Alba Nova Universitetscentrum,AlbaNova University Center. Stockholm Center for Physics, Astronomy and Biotechnology,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)

(creator_code:org_t)

2014-10-09
2014
Engelska.
Ingår i: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 14:21-22, s. 2498-2507

Relaterad länk:: http://dx.doi.org/10...; visa fler...; https://urn.kb.se/re...; https://doi.org/10.1...; https://urn.kb.se/re...; https://research.cha...; visa färre...

Abstract Ämnesord

Stäng

Global protein analysis of human gallbladder tissue is vital for identification of molecular regulators and effectors of its physiological activity. Here, we employed a genome-wide deep RNA sequencing analysis in 28 human tissues to identify the genes overrepresented in the gallbladder and complemented it with antibody-based immunohistochemistry in 48 human tissues. We characterized human gallbladder proteins and identified 140 gallbladder-specific proteins with an elevated expression in the gallbladder as compared to the other analyzed tissues. Five genes were categorized as enriched, with at least fivefold higher levels in gallbladder, 60 genes were categorized as group enriched with elevated transcript levels in gallbladder shared with at least one other tissue and 75 genes were categorized as enhanced with higher expression than the average expression in other tissues. We explored the localization of the genes within the gallbladder through cell-type specific antibody-based protein profiling and the subcellular localization of the genes through immunofluorescent-based profiling. Finally, we revealed the biological processes and metabolic functions carried out by these genes through the use of GO, KEGG Pathway, and HMR2.0 that is compilation of the human metabolic reactions. We demonstrated the results of the combined analysis of the transcriptomics and affinity proteomics.

Av författaren/redakt...: Kampf, Caroline; Mardinoglu, Adil ...; Fagerberg, Linn; Hallström, Björn ...; Danielsson, Ange ...; Nielsen, Jens; visa fler...; Pontén, Fredrik; Uhlén, Mathias; visa färre...

Om ämnet

Av lärosätet: Kungliga Tekniska Högskolan; Uppsala universitet; Chalmers tekniska högskola

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