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Recruitment of Epac...
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Alenkvist, IdaUppsala universitet,Institutionen för medicinsk cellbiologi
(author)
Recruitment of Epac2A to Insulin Granule Docking Sites Regulates Priming for Exocytosis
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-07-05
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American Diabetes Association,2017
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-336299
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-336299URI
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https://doi.org/10.2337/db17-0050DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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De två första författarna delar förstaförfattarskapet.
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Epac is a cAMP-activated guanine nucleotide exchange factor that mediates cAMP signaling in various types of cells, including -cells, where it is involved in the control of insulin secretion. Upon activation, the protein redistributes to the plasma membrane, but the underlying molecular mechanisms and functional consequences are unclear. Using quantitative high-resolution microscopy, we found that cAMP elevation caused rapid binding of Epac2A to the -cell plasma membrane, where it accumulated specifically at secretory granules and rendered them more prone to undergo exocytosis. cAMP-dependent membrane binding required the high-affinity cyclic nucleotide-binding (CNB) and Ras association domains, but not the disheveled-Egl-10-pleckstrin domain. Although the N-terminal low-affinity CNB domain (CNB-A) was dispensable for the translocation to the membrane, it was critical for directing Epac2A to the granule sites. Epac1, which lacks the CNB-A domain, was recruited to the plasma membrane but did not accumulate at granules. We conclude that Epac2A controls secretory granule release by binding to the exocytosis machinery, an effect that is enhanced by prior cAMP-dependent accumulation of the protein at the plasma membrane.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Gandasi, Nikhil RUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)nikga397
(author)
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Barg, Sebastian,1969-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)sebba507
(author)
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Tengholm, Anders,1971-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)andeteng
(author)
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Uppsala universitetInstitutionen för medicinsk cellbiologi
(creator_code:org_t)
Related titles
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In:Diabetes: American Diabetes Association66:10, s. 2610-26220012-17971939-327X
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