Search: L773:1043 3074 OR L773:1097 0347
> (2005-2009) >
Radiotherapy respon...
Radiotherapy response in oral squamous carcinoma cell lines : Evaluation of apoptotic proteins as prognostic factors
-
- Roberg, Karin, 1957- (author)
- Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet
-
- Jonsson, Ann-Charlotte (author)
- Linköpings universitet,Medicinsk radiofysik,Hälsouniversitetet
-
- Grénman, Reidar (author)
- Department of Otorhinolaryngology, Head and Neck Surgery, Central Hospital and University of Turku and Medical Biochemistry, University of Turku, Finland
-
show more...
-
- Norberg-Spaak, Lena, 1956- (author)
- Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet
-
show less...
-
(creator_code:org_t)
- 2007
- 2007
- English.
-
In: Head and Neck. - : Wiley. - 1043-3074 .- 1097-0347. ; 29:4, s. 325-334
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background. In this study, we investigated the importance of apoptosis for cell death after radiotherapy, and whether the expression of pro- and anti-apoptotic proteins has any correlation to the radiosensitivity.Methods. Three oral squamous cell carcinoma cell lines, UT-SCC-2, UT-SCC-9 and UT-SCC-24A, were subjected to radiotherapy. After irradiation, viable and dead cells were counted to determine radiation sensitivity and apoptosis was analyzed by measurement of caspase-3 activity. The expressions of pro- and anti-apoptotic proteins were assessed using western blot analyses.Results and Conclusion. After irradiation, apoptotic morphology and caspase-3 activity were only detected in cell lines exhibiting high or moderate radiosensitivity. Western blot analysis indicates that survivin, epidermal growth factor receptor, cyclooxygenase-2, and Bcl-xL are critical components in irradiation resistance of the investigated cell lines. Moreover, our results suggest that apoptotic cell death and the balance between pro- and anti-apoptotic proteins are of importance for the outcome of radiotherapy.
Keyword
- Apoptosis; oral tumors; predictive markers; radiotherapy; radiosensitivity
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database