Detection of Helicobacter ganmani-Like 16S rDNA in Pediatric Liver Tissue

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Fältnamn	Indikatorer	Metadata
000		04011naa a2200385 4500
001		oai:lup.lub.lu.se:ade0d92a-21bc-4a2c-b301-ac2320964a92
003		SwePub
008		160401s2004 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://lup.lub.lu.se/record/1324252 URI
024	7	a https://doi.org/10.1111/j.1083-4389.2004.00266.x2 DOI
040		a (SwePub)lu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Tolia, Vasundhara4 aut
245	1 0	a Detection of Helicobacter ganmani-Like 16S rDNA in Pediatric Liver Tissue
264	1	b Wiley,c 2004
338		a electronic2 rdacarrier
520		a Background. To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis.Materials and methods. Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects with no evidence of preexisting liver disease were examined for the presence of Helicobacter species by a genus-specific seminested polymerase chain reaction (PCR) assay. PCRproducts of positive samples were further characterized by denaturing gradient gel electrophoresis (DGGE) and DNA-sequence analysis. Based on those results, a seminested PCR assay for H. ganmani was developed and applied to the samples.Results. On analysis, 40/61 patient samples were positive in the genus-specific Helicobacter PCR and 4/10 from the control group. The nucleotide sequences of 16S rDNA fragments were 99100 similar to mainly Helicobacter sp. liver and H. ganmani. PCR-products similar to H. canis and H. bilis were also found. The 16S rDNAs of control specimens showed similarity to Helicobacter sp. liver. In the H. ganmani-specific PCR analysis 19 patients, but none of the controls, were positive.Conclusions. Amplified Helicobacter 16S rDNAs were related to Helicobacter sp. liver or H. ganmani in liver biopsy specimens of pediatric patients. The possible significance of Helicobacter species in pediatric liver diseases needs to be evaluated further in prospective studies.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
700	1	a Nilsson, Hans-Olofu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mmb-hon
700	1	a Boyer, Kimberly4 aut
700	1	a Wuerth, Anne4 aut
700	1	a Abu Al-Soud, Waleedu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mmb-wab
700	1	a Rabah, Raja4 aut
700	1	a Wadström, Torkelu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mmb-twa
710	2	a Avdelningen för medicinsk mikrobiologib Institutionen för laboratoriemedicin4 org
773	0	t Helicobacterd : Wileyg 9:5, s. 460-468q 9:5<460-468x 1083-4389x 1523-5378
856	4	u https://portal.research.lu.se/files/2729404/624309.pdfx primaryx freey FULLTEXT
856	4	u http://dx.doi.org/10.1111/j.1083-4389.2004.00266.xy FULLTEXT
856	4	u https://lup.lub.lu.se/search/files/2729404/624309.pdf
856	4 8	u https://lup.lub.lu.se/record/132425
856	4 8	u https://doi.org/10.1111/j.1083-4389.2004.00266.x

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Av författaren/redakt...: Tolia, Vasundhar ...; Nilsson, Hans-Ol ...; Boyer, Kimberly; Wuerth, Anne; Abu Al-Soud, Wal ...; Rabah, Raja; visa fler...; Wadström, Torkel; visa färre...

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