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Nucleus pulposus-induced apoptosis in dorsal root ganglion following experimental disc herniation in rats
- Murata, Y. (författare)
-
- Nannmark, Ulf, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Rydevik, Björn, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
- visa fler...
- Takahashi, K. (författare)
- visa färre...
- (creator_code:org_t)
- 2006
- 2006
- Engelska.
- Ingår i: Spine. - 1528-1159. ; 31:4, s. 382-90
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- STUDY DESIGN: The mechanisms behind the formation of a characteristic tissue reaction at the surface of the dorsal root ganglion (DRG) exposed to nucleus pulposus was studied with special reference to apoptosis using electron microscopy and immunohistochemistry in rats. OBJECTIVES: To study the mechanism of the characteristic tissue reaction at the surface of the DRG exposed to nucleus pulposus. SUMMARY OF BACKGROUND DATA: Recently, it was observed that local application of nucleus pulposus may induce a characteristic tissue reaction at the surface of the DRG. This change occurred as early as 1 day after the application of nucleus pulposus. METHODS.: Herniation of nucleus pulposus was created in the L4-L5 disc in rats. The L4 DRG were resected 3 and 24 hours after surgery. The sections of the specimens were observed using light and electron microscopy. The sections were processed for immunohistochemistry using antibodies to single-stranded DNA (ssDNA), caspase 3, and tumor necrosis factor alpha (TNF). RESULTS: There were typical changes of the cell nuclei observed by light and electron microscopy, especially those of the small-sized cells, in the DRG 24 hours after application of nucleus pulposus, indicating the presence of apoptosis. The presence of ssDNA, caspase 3, and TNF further enhanced the impression that there was apoptosis in the DRG. Nucleus pulposus induced apoptosis in the DRG at the site of application within as little as 24 hours. CONCLUSIONS: Nucleus pulposus herniated from the disc induced apoptosis in at the surface of the DRG exposed to nucleus pulpous as early as 24 hours after exposure.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Animals
- *Apoptosis
- Biological Markers/metabolism
- Caspase 3
- Caspases/metabolism
- Cell Nucleus/ultrastructure
- DNA
- Single-Stranded/analysis
- Disease Models
- Animal
- Ganglia
- Spinal/chemistry/metabolism/*pathology
- Immunohistochemistry
- Intervertebral Disk/metabolism/*pathology/surgery
- Intervertebral Disk Displacement/complications/*pathology
- Laminectomy
- Lumbar Vertebrae
- Rats
- Sprains and Strains
- Tumor Necrosis Factor-alpha/metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
- Murata, Y.
- Nannmark, Ulf, 1 ...
- Rydevik, Björn, ...
- Takahashi, K.
- Olmarker, Kjell, ...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Kirurgi
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- Göteborgs universitet
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