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Sökning: L773:1531 2267 OR L773:1094 8341 > (2015-2019) > Identification of s...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04532naa a2200433 4500 | |
| 001 | oai:DiVA.org:his-11419 | |
| 003 | SwePub | |
| 008 | 150825s2015 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-114192 URI |
| 024 | 7 | a https://doi.org/10.1152/physiolgenomics.00130.20142 DOI |
| 040 | a (SwePub)his | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Holmgren, Gustav,d 1983-u Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden,Bioinformatics4 aut0 (Swepub:his)holq |
| 245 | 1 0 | a Identification of stable reference genes in differentiating human pluripotent stem cells |
| 264 | 1 | b American Physiological Society,c 2015 |
| 338 | a print2 rdacarrier | |
| 520 | a Reference genes, often referred to as housekeeping genes (HKGs), are frequently used to normalize gene expression data based on the assumption that they are expressed at a constant level in the cells. However, several studies have shown that there may be a large variability in the gene expression levels of HKGs in various cell types. In a previous study, employing human embryonic stem cells (hESCs) subjected to spontaneous differentiation, we observed that the expression of commonly used HKG varied to a degree that rendered them inappropriate to use as reference genes under those experimental settings. Here we present a substantially extended study of the HKG signature in human pluripotent stem cells (hPSC), including nine global gene expression datasets from both hESC and human induced pluripotent stem cells (hiPSCs), obtained during directed differentiation towards endoderm-, mesoderm-, and ectoderm derivatives. Sets of stably expressed genes were compiled and a handful of genes (e.g., EID2, ZNF324B, CAPN10, and RABEP2) were identified as generally applicable reference genes in hPSCs across all cell lines and experimental conditions. The stability in gene expression profiles was confirmed by quantitative PCR (RT-qPCR) analysis. Taken together, the current results suggest that differentiating hPSCs have a distinct HKG signature, which in some aspects is different from somatic cell types, and underscore the necessity to validate the stability of reference genes under the actual experimental setup used. In addition, the novel putative HKGs identified in this study can preferentially be used for normalization of gene expression data obtained from differentiating hPSCs. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
| 653 | a Differentiation | |
| 653 | a Endogenous controls; Gene expression | |
| 653 | a Housekeeping genes | |
| 653 | a Human pluripotent stem cells | |
| 653 | a Normalization | |
| 653 | a Reference genes | |
| 653 | a Bioinformatik | |
| 653 | a Bioinformatics | |
| 700 | 1 | a Ghosheh, Nidalu Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Department of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden,Bioinformatics4 aut0 (Swepub:his)ghon |
| 700 | 1 | a Zeng, Xianminu Buck Institute for Research on Aging, Buck Institute, Novato, California, USA4 aut |
| 700 | 1 | a Bogestål, Yaldau Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatics4 aut0 (Swepub:his)bogy |
| 700 | 1 | a Sartipy, Peteru Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,AstraZeneca Research and Development, Global Medicines Development, Cardiovascular and Metabolic Diseases Global Medicines Development Unit, Mölndal, Sweden,Bioinformatics4 aut0 (Swepub:his)sarp |
| 700 | 1 | a Synnergren, Janeu Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatics4 aut0 (Swepub:his)synj |
| 710 | 2 | a Högskolan i Skövdeb Institutionen för biovetenskap4 org |
| 773 | 0 | t Physiological Genomicsd : American Physiological Societyg 47:6, s. 232-239q 47:6<232-239x 1094-8341x 1531-2267 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11419 |
| 856 | 4 8 | u https://doi.org/10.1152/physiolgenomics.00130.2014 |
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