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PtdIns(4,5)P2 is no...
PtdIns(4,5)P2 is not required for secretory granule docking
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- Omar-Hmeadi, Muhmmad (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Gandasi, Nikhil R (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Barg, Sebastian, 1969- (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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(creator_code:org_t)
- 2018-04-24
- 2018
- Engelska.
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Ingår i: Traffic. - : Wiley. - 1398-9219 .- 1600-0854. ; 19:6, s. 436-445
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Abstract
Ämnesord
Stäng
- Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution total internal reflection imaging of EGFP-labeled PtdIns markers or syntaxin-1 at secretory granule release sites in live insulin-secreting cells. In intact cells, PtdIns markers distributed evenly across the plasma membrane with no preference for granule docking sites. In contrast, syntaxin-1 was found clustered in the plasma membrane, mostly beneath docked granules. We also observed rapid accumulation of syntaxin-1 at sites where granules arrived to dock. Acute depletion of plasma membrane phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P-2) by recruitment of a 5-phosphatase strongly inhibited Ca2+-dependent exocytosis, but had no effect on docked granules or the distribution and clustering of syntaxin-1. Cell permeabilization by -toxin or formaldehyde-fixation caused PtdIns marker to slowly cluster, in part near docked granules. In summary, our data indicate that PtdIns(4,5)P-2 accelerates granule priming, but challenge a role of PtdIns in secretory granule docking or clustering of syntaxin-1 at the release site.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- exocytosis
- insulin
- live cell imaging
- phosphoinositides
- PtdIns(4
- 5)P-2
- syntaxin clustering
- vesicle docking
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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