Gli1 is not required for Pdgfralpha expression during mouse embryonic development

• Pdgfra is expressed in the mesenchyme of multiple organs during embryonic development and Pdgfrα is involved in cell proliferation, differentiation, migration, and apoptosis in many tissues. A fine-tuned regulation of gene transcription is required to achieve these effects. To investigate if the Shh signaling pathway is involved in the tightly regulated Pdgfra expression during embryogenesis, we systematically compared Gli1 and Pdgfrα mRNA expression patterns in vivo from mouse embryonic day 9.5 to 14.5. We found that an initial partly overlapping expression of Gli1 and Pdgfrα in the mesenchyme of foregut and somites was changed to different expression patterns when the mesenchyme differentiated into specialized structures such as intestinal villi and chondrocytes. Gli1 and Pdgfra were also expressed differently in the developing lung, heart, central nervous system, skin, tooth, and eye. Importantly, neither Pdgfrα mRNA patterns nor levels were altered in Ihh mutant embryos although Gli1 and Ptc mRNA levels were dramatically reduced. Our results demonstrate that Gli1 is not required to induce Pdgfra expression during embryonic bone development, and are consistent with previous findings that Pdgfrα and Hh pathways serve different functions in, e.g., bone, gut, and lung development. However, we cannot exclude the possibility that Glis can have more complex regulatory effects on Pdgfra gene activity, nor can we exclude such effects in pathological conditions.

Nyckelord

Animals

Base Sequence

Binding Sites

Conserved Sequence

Embryonic Development/genetics

Gene Expression Regulation; Developmental

Kruppel-Like Transcription Factors

Mice

Mice; Inbred Strains

Mutagenesis

RNA; Messenger/genetics

Receptor; Platelet-Derived Growth Factor alpha/*genetics

Research Support; Non-U.S. Gov't

Transcription Factors/*metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)