Search: WFRF:(Aili Daniel)
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Colorimetric Protei...
Colorimetric Protein Sensing by Controlled Assembly of Gold Nanoparticles Functionalized with Synthetic Receptors
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- Aili, Daniel, 1977- (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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- Selegård, Robert (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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- Baltzer, Lars (author)
- Uppsala universitet,Institutionen för biokemi och organisk kemi,Division of Organic Chemistry, Department of Biochemistry and Organic Chemistry, BMC, Box 576, Uppsala University, SE-751 23 Uppsala, Sweden
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- Enander, Karin (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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- Liedberg, Bo (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 5:21, s. 2445-2452
- Related links:
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http://urn.kb.se/res...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn2+-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximate to 10 nM and approximate to 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- bioassays
- gold
- helical structures
- hybrid materials
- nanoparticles
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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