WFRF:(Areschoug Thomas)
Search: WFRF:(Areschoug Thomas) > (2010-2014) > Human Siglec-5 Inhi...
- 4 of 4
- Previous record
- Next record
- To hitlist
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04482naa a2200385 4500 | |
| 001 | oai:lup.lub.lu.se:090925b4-6cca-4183-b666-3fa7ea56da6c | |
| 003 | SwePub | |
| 008 | 160401s2011 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/21685722 URI |
| 024 | 7 | a https://doi.org/10.1074/jbc.M111.2517282 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Nordström, Theréseu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mikr-tno |
| 245 | 1 0 | a Human Siglec-5 Inhibitory Receptor and Immunoglobulin A (IgA) Have Separate Binding Sites in Streptococcal {beta} Protein. |
| 264 | 1 | c 2011 |
| 520 | a Sialic acid-binding immunoglobulin-like lectins (Siglecs) are receptors believed to be important for regulation of cellular activation and inflammation. Several pathogenic microbes bind specific Siglecs via sialic acid-containing structures at the microbial surface, interactions that may result in modulation of host responses. Recently, it was shown that the group B Streptococcus (GBS) binds to human Siglec-5 (hSiglec-5), an inhibitory receptor expressed on macrophages and neutrophils, via the IgA-binding surface β protein, providing the first example of a protein/protein interaction between a pathogenic microbe and a Siglec. Here we show that the hSiglec-5-binding part of β resides in the N-terminal half of the protein, which also harbors the previously determined IgA-binding region. We constructed bacterial mutants expressing variants of the β protein with non-overlapping deletions in the N-terminal half of the protein. Using these mutants and recombinant β fragments, we showed that the hSiglec-5-binding site is located in the most N-terminal part of β (B6N region; amino acids 1-152) and that the hSiglec-5- and IgA-binding domains in β are completely separate. We showed with BIAcore(TM) analysis that tandem variants of the hSiglec-5- and IgA-binding domains bind to their respective ligands with high affinity. Finally, we showed that the B6N region, but not the IgA-binding region of β, triggers recruitment of the tyrosine phosphatase SHP-2 to hSiglec-5 in U937 monocytes. Taken together, we have identified and isolated the first microbial non-sialic acid Siglec-binding region that can be used as a tool in studies of the β/hSiglec-5 interaction. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng |
| 700 | 1 | a Movert, Elinu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-emt |
| 700 | 1 | a Olin, Andersu Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)medk-ano |
| 700 | 1 | a Ali, Syed R4 aut |
| 700 | 1 | a Nizet, Victor4 aut |
| 700 | 1 | a Varki, Ajit4 aut |
| 700 | 1 | a Areschoug, Thomasu Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mmb-tar |
| 710 | 2 | a Avdelningen för medicinsk mikrobiologib Institutionen för laboratoriemedicin4 org |
| 773 | 0 | t Journal of Biological Chemistryg 286:39, s. 33981-33991q 286:39<33981-33991x 1083-351X |
| 856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/21795693?dopt=Abstracty FULLTEXT |
| 856 | 4 | u http://dx.doi.org/10.1074/jbc.M111.251728y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/2168572 |
| 856 | 4 8 | u https://doi.org/10.1074/jbc.M111.251728 |
Find in a library
- Journal of Biological Chemistry (Search for host publication in LIBRIS)
To the university's database
- 4 of 4
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Nordström, Theré ...
- Movert, Elin
- Olin, Anders
- Ali, Syed R
- Nizet, Victor
- Varki, Ajit
- show more...
- Areschoug, Thoma ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Infectious Medic ...
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Microbiology in ...
- Articles in the publication
- Journal of Biolo ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
