Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

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Fältnamn	Indikatorer	Metadata
000		05171naa a2200457 4500
001		oai:DiVA.org:liu-165348
003		SwePub
008		200427s2020 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1653482 URI
024	7	a https://doi.org/10.3390/toxins120301962 DOI
040		a (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Oliveira, Flaviou Univ Porto, Portugal4 aut
245	1 0	a Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
264		c 2020-03-20
264	1	b MDPI,c 2020
338		a electronic2 rdacarrier
500		a Funding Agencies\|Portuguese Science Foundation (Fundacao para a Ciencia e a Tecnologia, FCT)Portuguese Foundation for Science and Technology [SFRH_BPD_103683_2014]; NORTE 2020, Portugal 2020 [UID/Multi/04423/2013, PTDC/ASP-PES/31762/2017]; European Union through the ERDFEuropean Union (EU); FCTPortuguese Foundation for Science and Technology; MINECO/FEDER, UE [AGL2015-64558-R]; FPISpanish Government [BES-2016-078773]
520		a Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 x 10(5) cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.
650	7	a NATURVETENSKAPx Biologix Annan biologi0 (SwePub)106992 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Other Biological Topics0 (SwePub)106992 hsv//eng
653		a Mytilus galloprovincialis; toxic cyanobacteria; microcystin; cylindrospermopsin; ecotoxicology; shotgun proteomics
700	1	a Diez-Quijada, Leticiau Univ Seville, Spain4 aut
700	1	a Turkina, Maria V,d 1973-u Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten4 aut0 (Swepub:liu)martu07
700	1	a Morais, Joaou Univ Porto, Portugal4 aut
700	1	a Felpeto, Aldo Barreirou Univ Porto, Portugal4 aut
700	1	a Azevedo, Joanau Univ Porto, Portugal4 aut
700	1	a Jos, Angelesu Univ Seville, Spain4 aut
700	1	a Camean, Ana M.u Univ Seville, Spain4 aut
700	1	a Vasconcelos, Vitoru Univ Porto, Portugal; Univ Porto, Portugal4 aut
700	1	a Martins, Jose Carlosu Univ Porto, Portugal4 aut
700	1	a Campos, Alexandreu Univ Porto, Portugal4 aut
710	2	a Univ Porto, Portugalb Univ Seville, Spain4 org
773	0	t Toxinsd : MDPIg 12:3q 12:3x 2072-6651
856	4	u https://liu.diva-portal.org/smash/get/diva2:1426750/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4	u https://www.mdpi.com/2072-6651/12/3/196/pdf
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-165348
856	4 8	u https://doi.org/10.3390/toxins12030196

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Av författaren/redakt...: Oliveira, Flavio; Diez-Quijada, Le ...; Turkina, Maria V ...; Morais, Joao; Felpeto, Aldo Ba ...; Azevedo, Joana; visa fler...; Jos, Angeles; Camean, Ana M.; Vasconcelos, Vit ...; Martins, Jose Ca ...; Campos, Alexandr ...; visa färre...

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